DNA sequence analysis indicates human origin of rotavirus and hepatitis A virus strains from western Colombia
Keywords:
rotavirus, hepatitis A virus, water pollution, phylogeny, diarrhea
Abstract
Introduction. Quibdó, the capital of Chocó Province, is one of the poorest cities in Colombia. Enteric viruses such as rotavirus and hepatitis A virus was found to occur commonly in city drinking water and indicated poor water quality and high risk of becoming infected. The source of these viruses was unknown, but humans and cattle were suspect sources.Objective. City water was assessed to determine source and persistence of diarrhea and hepatitis among the human populations in the environs of Quibdó.
Material and methods. Four thousand liters of water were collected, filtered by tangential ultrafiltration and centrifuged in Centriprep Ultracel YM-50 tubes. Sixty samples of untreated and 20 of treated water were probed by RT-PCR.
Results. Six samples were positive for rotavirus and 2 for hepatitis A virus in both, treated and non treated water. DNA sequence analysis identified the presence of human G2 rotavirus and human hepatitis A virus.
Conclusion. The evidence indicated a high level of contamination with pathogenic viruses in consumable water sources in Quibdó, Colombia.
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References
1. Bosch A, Guix S, Sano D, Pintó R. New tools for the study and direct surveillance of viral pathogens in water. Curr Opin Biotechnol. 2008;19:295-301.
2. Procuraduría General de la Nación, Ministerio del Medio Ambiente, UNICEF. Agua para la vida, Colombia y el mundo. Panorama nacional: Los niños, el agua y el ambiente sano. La infancia, el agua y el saneamiento básico en los planes de desarrollo departamentales y municipales. Primera edición. Bogotá, D.C.: CMYK Impresiones Ltda.; 2006. p. 18-29.
3. Eltiempo.com/archivo. La tragedia del Chocó. El Tiempo. Enero 2005; Editorial Opinión. Fecha de consulta: 15 de febrero de 2007. Disponible en: http://www.eltiempo.com/archivo/documento/MAM-1674069
4. Byun K, Kim J, Song K, Baek L, Song J, Park S, et al. Molecular studies: Hepatitis A virus and hepatectomy. Molecular epidemiology of hepatitis A in Korea. J Gastroenterol Hepatol. 2001;16:519-24.
5. Cuthbert JA. Hepatitis A: Old and new. Clin Microbiol Rev. 2001;14:38-58.
6. Costa-Mattioli M, Cristina J, Romero H, Pérez-Bercof R, Casane D, Colina R, et al. Molecular evolution of hepatitis A virus: A new classification based on the complete Vp1 protein. J Virol. 2002;76:9516-25.
7. Fiore A. Hepatitis A transmitted by food. Clin Infect Dis. 2004;38:705-15.
8. Hernández OA. El agua de aquí, es la que cae del cielo. Fecha de consulta: 15 de febrero de 2007. Disponible en: http://www.latarde.com/2007/2/15/nac1.htm
9. Martínez L, Matiz A, Trespalacios AA, Ajami N, Mora CI, Serrano P, et al. Etiología de la enfermedad diarreica aguda en niños menores de 5 años en la población de Quibdó. El calicivirus, un nuevo hallazgo. Pediatría. 2005;40:43-52.
10. Rubio MP, Castro J, Gutiérrez E, Tanaka J. Seroprevalencia de la hepatitis A y varicela en Bogotá, Colombia. Revista Panamericana de Infectología. 2006. Fecha de consulta: 15 de febrero de 2007. Disponible en: http://encolombia.com/medicina/ infectologia/revista panadeinfev4-1-investigaseroprevaha.htm
11. Rodríguez C. Actualización sobre hepatitis virales: Etiología, patogenia, diagnóstico microbiológico y prevención. Rev Cubana Med Gen Integr. 2000;16:574-85.
12. Costa-Mattioli M, Di Napoli A, Ferré V, Billaudel S, Pérez R, Cristina J. Genetic diversity of hepatitis A virus. J Gen Virol. 2003;84:3191-201.
13. Deng M, Day S, Cliver D. Detection of hepatitis a virus in environmental samples by antigen-capture PCR. Appl Environ Microbiol. 1994;60:1927-33.
14. Brooks H, Gersberg R, Dhar A. Detection and quantification of hepatitis A virus in seawater via real-time RT-PCR. J Virol Methods. 2005;127:109-18.
15. Gouvea V, Glass R, Woods P, Taniguchi K, Clark H, Forrester B, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol. 1990;28:276-82.
16. Dubois E, Hennechart C, Merle G, Burger C, Hmila N, Ruelle S, et al. Detection and quantification by real time RT-PCR of hepatitis A virus from inoculated tap waters, salad vegetables and soft fruits: Characterization of the method performances. Int J Food Microbiol. 2007;117:141-9.
17. Espinosa A, Mazari-Hiriart M, Espinosa R, Maruri-Avidal L, Méndez E, Arias C. Infectivity and genome persistence of rotavirus and astrovirus in groundwater and surface water. Water Res. 2008;42:2618-28.
18. Deetz TR, Smith EM, Goyal SM, Gerba CP, Vollet JJ, Tsai L, et al. Occurrence of rota-and enteroviruses in drinking and environmental water in a developing nation. Water Res. 1984;18:567-71.
19. Keswick BH, Gerba CP, DuPont HL, Rose JB. Detection of enteric viruses in treated drinking water. Appl Environ Microbiol. 1984;47:1290-4.
20. Gutiérrez M, Alvarado M, Martínez E, Ajami N. Presence of viral proteins in drinkable water-sufficient condition to consider water a vector of viral transmission? Water Res. 2007;41:373-8.
21. Haramoto E, Katayama H, Utagawa E, Ohgaki S. Development of sample storage methods for detecting enteric viruses in environmental water. J Virol Methods. 2008;151:1-6.
22. Duan Z, Li D, Zhang Q, Liu N, Huang C, Jiang X, et al. Novel human rotavirus of genotype G5P(6) identified in a stool specimen from a Chinese girl with diarrhea. J Clin Microbiol. 2007;45:1614-7.
2. Procuraduría General de la Nación, Ministerio del Medio Ambiente, UNICEF. Agua para la vida, Colombia y el mundo. Panorama nacional: Los niños, el agua y el ambiente sano. La infancia, el agua y el saneamiento básico en los planes de desarrollo departamentales y municipales. Primera edición. Bogotá, D.C.: CMYK Impresiones Ltda.; 2006. p. 18-29.
3. Eltiempo.com/archivo. La tragedia del Chocó. El Tiempo. Enero 2005; Editorial Opinión. Fecha de consulta: 15 de febrero de 2007. Disponible en: http://www.eltiempo.com/archivo/documento/MAM-1674069
4. Byun K, Kim J, Song K, Baek L, Song J, Park S, et al. Molecular studies: Hepatitis A virus and hepatectomy. Molecular epidemiology of hepatitis A in Korea. J Gastroenterol Hepatol. 2001;16:519-24.
5. Cuthbert JA. Hepatitis A: Old and new. Clin Microbiol Rev. 2001;14:38-58.
6. Costa-Mattioli M, Cristina J, Romero H, Pérez-Bercof R, Casane D, Colina R, et al. Molecular evolution of hepatitis A virus: A new classification based on the complete Vp1 protein. J Virol. 2002;76:9516-25.
7. Fiore A. Hepatitis A transmitted by food. Clin Infect Dis. 2004;38:705-15.
8. Hernández OA. El agua de aquí, es la que cae del cielo. Fecha de consulta: 15 de febrero de 2007. Disponible en: http://www.latarde.com/2007/2/15/nac1.htm
9. Martínez L, Matiz A, Trespalacios AA, Ajami N, Mora CI, Serrano P, et al. Etiología de la enfermedad diarreica aguda en niños menores de 5 años en la población de Quibdó. El calicivirus, un nuevo hallazgo. Pediatría. 2005;40:43-52.
10. Rubio MP, Castro J, Gutiérrez E, Tanaka J. Seroprevalencia de la hepatitis A y varicela en Bogotá, Colombia. Revista Panamericana de Infectología. 2006. Fecha de consulta: 15 de febrero de 2007. Disponible en: http://encolombia.com/medicina/ infectologia/revista panadeinfev4-1-investigaseroprevaha.htm
11. Rodríguez C. Actualización sobre hepatitis virales: Etiología, patogenia, diagnóstico microbiológico y prevención. Rev Cubana Med Gen Integr. 2000;16:574-85.
12. Costa-Mattioli M, Di Napoli A, Ferré V, Billaudel S, Pérez R, Cristina J. Genetic diversity of hepatitis A virus. J Gen Virol. 2003;84:3191-201.
13. Deng M, Day S, Cliver D. Detection of hepatitis a virus in environmental samples by antigen-capture PCR. Appl Environ Microbiol. 1994;60:1927-33.
14. Brooks H, Gersberg R, Dhar A. Detection and quantification of hepatitis A virus in seawater via real-time RT-PCR. J Virol Methods. 2005;127:109-18.
15. Gouvea V, Glass R, Woods P, Taniguchi K, Clark H, Forrester B, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol. 1990;28:276-82.
16. Dubois E, Hennechart C, Merle G, Burger C, Hmila N, Ruelle S, et al. Detection and quantification by real time RT-PCR of hepatitis A virus from inoculated tap waters, salad vegetables and soft fruits: Characterization of the method performances. Int J Food Microbiol. 2007;117:141-9.
17. Espinosa A, Mazari-Hiriart M, Espinosa R, Maruri-Avidal L, Méndez E, Arias C. Infectivity and genome persistence of rotavirus and astrovirus in groundwater and surface water. Water Res. 2008;42:2618-28.
18. Deetz TR, Smith EM, Goyal SM, Gerba CP, Vollet JJ, Tsai L, et al. Occurrence of rota-and enteroviruses in drinking and environmental water in a developing nation. Water Res. 1984;18:567-71.
19. Keswick BH, Gerba CP, DuPont HL, Rose JB. Detection of enteric viruses in treated drinking water. Appl Environ Microbiol. 1984;47:1290-4.
20. Gutiérrez M, Alvarado M, Martínez E, Ajami N. Presence of viral proteins in drinkable water-sufficient condition to consider water a vector of viral transmission? Water Res. 2007;41:373-8.
21. Haramoto E, Katayama H, Utagawa E, Ohgaki S. Development of sample storage methods for detecting enteric viruses in environmental water. J Virol Methods. 2008;151:1-6.
22. Duan Z, Li D, Zhang Q, Liu N, Huang C, Jiang X, et al. Novel human rotavirus of genotype G5P(6) identified in a stool specimen from a Chinese girl with diarrhea. J Clin Microbiol. 2007;45:1614-7.
How to Cite
1.
Gutiérrez MF, Moreno S, Alvarado MV, Bermúdez A. DNA sequence analysis indicates human origin of rotavirus and hepatitis A virus strains from western Colombia. Biomed. [Internet]. 2009 Jun. 1 [cited 2025 Apr. 4];29(2):209-17. Available from: https://revistabiomedicaorg.biteca.online/index.php/biomedica/article/view/23
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