Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia
Abstract
Introduction: There is a high incidence and prevalence of dengue in the department of Cundinamarca, and recently Aedes aegypti, the main vector of dengue virus (DENV), was detected in some of its rural areas.
Objective: To evaluate viral transovarial transmission in larvae and pupae collected in rural areas of the municipality of Anapoima, Cundinamarca.
Materials and methods: Live larvae and pupae were collected from 53 homes and later they were taken to the laboratory in Anapoima, where they were classified, pooled and frozen. In Bogotá, they were homogenized, RNA was extracted with Trizol™, and RT-PCR and conventional PCR were performed. The amplified products were analyzed on 2% agarose gels.
Results: In 54.7% of the houses we found A. aegypti in immature stages, and DENV-1 was the most frequent serotype. However, the simultaneous presence of DENV 1 and 2, DENV 1 and 3, DENV 1 and 4, and DENV 1, 2 and 3 serotypes was detected in some pools.
Conclusion: The results confirmed the natural vertical transmission of the virus in the rural area under study. These findings confirmed the vector capacity of A. aegypti, and partly explains the persistence of the virus in the region and the possibility of transmission by the vector during adulthood without having ingested infected blood. This situation increases the risk of DENV infection in Colombia and the need for prevention and control programs in all areas where the mosquito is present.
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References
Christopher R. Aedes aegypti (L.). The yellow fever mosquito. Its life history, bionomics and structure. New York: Cambridge University Press; 1960. p. 739.
Manrique P, Che-Mendoza A, Rizzo N, Arana B, Pilger D, Lenhart A, et al. Operational guide for assessing the productivity of Aedes aegypti breeding sites Geneva: World Health Organization; 2011. https://doi.org/10.2471/TDR.10. 978-924-1500371
Icaza J. El mosquito Aedes aegypti y el dengue en México. México: Bayer de México; 2003. p. 152.
Tilak R, Ray S, Tilak V, Mukherji S. Dengue, chikungunya…and the missing entity-Zika fever: A new emerging threat. Med J Armed Forces India. 2016;72:157-63. https://doi.org/10.1016/j.mjafi.2016.02.017
Padilla JC, Rojas DP, Sáenz-Gómez R. Dengue en Colombia: epidemiología de la reemergencia a la hiperendemia. Bogotá: Guías de Impresión Ltda.; 2012. p. 1-17.
Suárez N. Registro de altitud del Aedes aegypti en Colombia. Biomédica 1981;1:225. https://doi.org/10.7705/biomedica.v1i4.1809
Ruiz-López F, González-Mazo A, Vélez-Mira A, Gómez GF, Zuleta L, Uribe S, et al. Presencia de Aedes (Stegomyia) aegypti (Linnaeus, 1762) y su infección natural con el virus dengue en alturas no registradas para Colombia. Biomédica. 2016;36:303-8. https://doi.org/10.7705/biomedica. v36i2.3301
Olano V, Matiz M, Lenhart A, Cabezas L, Vargas S, Jaramillo J, et al. Schools as potential risk sites for vector-borne disease transmission: Mosquito vectors in rural schools in two municipalities in Colombia. J Am Mosq Control Assoc. 2015;31:212-22. https://doi.org/10.2987/moco- 31-03-212-222.1
Morales A. Aedes aegypti en zona rural del municipio de La Mesa (Cundinamarca) Colombia, S.A. Biomédica. 1981;1:223-4. https://doi.org/10.7705/biomedica.v1i4.1806
Pérez-Castro R, Castellanos J, Olano V, Matiz M, Jaramillo J, Vargas S, et al. Detection of all four serotypes in Aedes aegypti female mosquitoes collected in a rural area in Colombia. Mem Inst Oswaldo Cruz. 2016;111:233-40. https://doi.org/10.1590/0074-02760150363
Thenmozhi V, Tewari S, Manavalan R, Balasubramanian A, Gajanana A. Natural vertical transmission of dengue viruses in Aedes aegypti in southern India. Trans R Soc Trop Med Hyg. 2000;94:507.
Thenmozhi V, Hiriyan J, Tewari S, Samuel P, Paramasivan R, Rajendran R, et al. Natural vertical transmission of dengue virus in Aedes albopictus (Diptera: Culicidae) in Kerala, a southern Indian state. Jpn J Infect Dis. 2007;60:245-9.
Romero-Vivas C, Arango-Padilla P, Falconar A. Pupal productivity surveys to identify the key container habitats of Aedes aegypti (L) in Barranquilla, the principal seaport of Colombia. Ann Trop Med Parasitol. 2006;100(Suppl.1):S87-95. https://doi.org/10.1179/136485906X105543
Tinker M, Olano V. Ecología del Aedes aegypti en un pueblo de Colombia, Suramérica. Biomédica. 1993;13:5-14. https://doi.org/10.7705/biomedica.V13i1.2041
González R, Darsie JR Jr. Clave ilustrada para la deter-minación genérica de larvas de Culicidae de Colombia y el nuevo mundo. Bol Mus Entomol Univ Valle. 1996;4:21-37.
Tinker M, Stojanovich CJ. Identification of the pupae of receptacle breeding mosquitoes. Ann Entomol Soc Am. 1962;55:577-82.
Chien L, Liao T, Shu PY, Huang J, Gubler D, Chang G. Development of real time reverse transcriptase PCR assays to detect and serotype dengue viruses. J Clin Microbiol. 2006;44:1295-304. https://doi.org/10.1128/JCM.44.4.1295-1304.2006
Staley M, Dorman K, Bartholomay L, Fernández-Salas I, Farfán-Ale J, Loroño-Pino MA, et al. Universal primers for the amplification and sequence analysis of actin-1 from diverse mosquito species. J Am Mosq Control Assoc. 2010;26:214-8. https://doi.org/10.2987/09-5968.1
Grunnill M, Boots M. How important is vertical transmission of dengue viruses by mosquitoes (Diptera: Culicidae)? J Med Entomol. 2016;53:1-19. https://doi.org/10.1093/jme/tjv168
Khin M, Than K. Transovarial transmission of dengue 2 virus by Aedes aegypti in nature. Am J Trop. Med Hyg. 1983;32: 590-4. https://doi.org/10.4269/ajtmh.1983.32.590
Mbaika S, Lutomiah J, Chepkorir E, Mulwa F, Khayeka-Wandabwa C, Tigoi C, et al. Vector competence of Aedes aegypti in transmitting Chikungunya virus: Effects and implications of extrinsic incubation temperature on dissemi-nation and infection rates. Virol J. 2016;13:114. https://doi.org/10.1186/s12985-016-0566-7
Service M. Culicine mosquitoes (Culicinae). In: Medical Entomology for Students. Cambridge: Cambridge University Press; 2012. p. 52-80. https://doi.org/10.1017/CBO978051 1811012.007
Rey J, Lounibos P. Ecología de Aedes aegypti y Aedes albopictus en América y transmisión de enfermedades. Biomédica. 2015;35:177-85. https://doi.org/10.1590/S0120-41572015000200005
DANE. Conceptos básicos. Fecha de consulta: 31 de julio de 2016. Disponible en: http://www.dane.gov.co/files/inf_geo/4Ge_ConceptosBasicos.pdf
Bara J, Clark T. Remold susceptibility of larval Aedes aegypti and Aedes albopictus (Diptera: Culicidae) to dengue virus. J Med Entomol. 2013;50:179-84.
Joshi V, Mourya D, Sharma R. Persistence of dengue -3 virus through transovarial transmission passage in successive generation of Aedes aegypti mosquitoes. Am J Trop Med Hyg. 2002;67:158-61.
Barrera R. Recomendaciones para la vigilancia de Aedes aegypti. Biomédica. 2016;36:454-62. https://doi.org/10.7705/biomedica.v36i3.2892
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