Characteristics of new cell cultures derived from embryonic tissues of Aedes aegypti (Diptera: Culicidae).

Abstract

Introduction. Cell cultures from insects are a useful methodology in technological and biomedical studies.
Objective. The present work was aimed at obtaining and characterizing cell cultures derived from Aedes aegypti embryonic tissues.
Materials And Methods. Embryonated eggs were used for embryonic tissue explants in L-15/Grace and MMNP12 culture media, supplemented with 20% fetal bovine serum and a mixture of 1% antimycotic and antibiotics, at a pH ranging from 6.8 to 7.0. The incubation temperature was 28 degrees C; a CO2 atmosphere was not required.
Results. Cell growth was obtained in L-15/Grace medium three weeks after embryonic tissues explants. Six months were required for achieving a confluent monolayer. Twenty-eight serial cell subcultures were carried out from August 2003 to June 2004. Cell morphology was characterized as epithelial in the later subcultures. Karyotype morphometry as well as molecular and isozymatic profiles were established. The cultures were compared with adult samples from the species taken from the same colony and with cell lines derived from other insects.
Discussion And Conclusions. These cells are an important in vitro system in applied and basic research.