Evaluation of a modified capillary technique for immunocytochemistry of coverslip adhered cultures
Keywords:
immunocytochemistry, capillary technique, neuron culture, antibody
Abstract
In order to evaluate a simple and easy immunocytochemical method for cultured cell monolayers adhered to coverslips, we compared a minichamber technique with the conventional method using 24 well culture plates. lmmunocytochemical procedures were carried out on adult mice dorsal root ganglia cultures for fluorescence and peroxidase detection of neurofilament, S-100 protein or rabies virus. lntensity and especificity were similar using both techniques. Primary and secondary antibodies and enzymes volumes saving was 92% with similar reactivity and no increase in processing time of samples. This tecnique only requires materials routinely found in the labratory.Downloads
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How to Cite
1.
Quiroga NY, Martínez M, Castellanos JE, Hurtado H. Evaluation of a modified capillary technique for immunocytochemistry of coverslip adhered cultures. Biomed. [Internet]. 1999 Sep. 1 [cited 2025 Apr. 4];19(3):245-9. Available from: https://revistabiomedicaorg.biteca.online/index.php/biomedica/article/view/1029
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Published
1999-09-01
Issue
Section
Technical note
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