Recovery of Cryptococcus neoformans var. gattii serotype C from almond trees detritus

Abstract

During 1996 to 1997 a study conducted to search for the habitat of C. neoformans var. gattii was carried out in Cúcuta, due to the high frequency of clinical cases caused by this variety. From a total of 68 almond trees (Terrninalia catappa), two were positive for C. neoforrnans var. gattii serotype C. The aim of the present study was to follow-up the 2 positive trees in order to evaluate the permanence of the yeast in different months of the year. Samples were extracted with PBS supplemented with antibiotics and were plated in selective media. Species were assigned based on macro and microscopic morphology and biochemical tests. Variety and serotype was also determined with standardized techniques. From the two positive almond trees, samples were taken during 26 months and additionally 9 new almond trees were screened. From 160 samples, 31 (19,3%) were positive for C. neoforrnans var. gatfiiserotype C as well as 1 of the 9 extra trees.These findings confirmed that almond trees (Terrninalia catappa) could be a intermediate habitat for the var. gattii. In addition, that C. neoformans var. gattii serotype C could be present during the whole year in the area.