Eggshell membrane treatment for the healing of superficial open wounds in mice
Abstract
Introduction: Eggshell membranes offer beneficial properties for tissue regeneration and their biomedical applications are important.
Objective: To demonstrate the effectiveness of hen unfertilized eggshell membranes in the treatment of superficial open wounds in mice compared to the conventional procedure.
Materials and methods: 15-mm linear wounds were made on the back of 10 male CF-1 mice. The mice were divided into four groups. One group did not receive any treatment and the other three were given conventional treatment, eggshell membranes dressing, and eggshell membranes powder. The evolution of the wounds was registered by photographs. We measured wounds length and healing time and calculated the length reduction rate and the percentage of healing. The healing percentages were analyzed by ANOVA with a post hoc Dunnett test (p<0.05).
Results: The treatments with eggshell membranes and membrane powder had a woundlength reduction rate of 1.009 and 1.020 mm/day, greater than the conventional treatment, of 0.852 mm/day, and a healing time of 12 days, i.e., less than the 16-day conventional treatment. The statistical analysis showed significant differences between eggshell membrane treatments and the conventional treatment.
Conclusions: Eggshell membrane dressing and eggshell membrane powder applied to open superficial wounds in mice were more effective than the conventional treatment in regenerating injured tissue in mice.
Downloads
References
Kanitakis J. Anatomy, histology and immunohistochemistry of normal human skin. Eur J Dermatol. 2002;12:390-9.
Trott AT, Baena J. Heridas y cortes: tratamiento y sutura de urgencia. Madrid: Elsevier; 2007.
Salem. C, Pérez JA, Henning E, Uherek F, Schultz C, Butte JM, et al. Heridas. Conceptos generales. Cuad Cir. 2018;14:90-9. https://doi.org/10.4206/cuad.cir.2000.v14n1-15
Bosch Á. Las heridas y su tratamiento. Offarm. 2001;20:89-92.
Rodríguez R, González JH. Métodos alternativos para el tratamiento de pacientes con heridas infectadas. MEDISAN. 2011;15:503-14.
Baláž M. Eggshell membrane biomaterial as a platform for applications in materials science. Acta Biomater. 2014;10:3827-43. https://doi.org/10.1016/j.actbio.2014.03.020
Torres FG, Troncoso OP, Piaggio F, Hijar A. Structure–property relationships of a biopolymer network: The eggshell membrane. Acta Biomater. 2010;6:3687-93. https://doi.org/10.1016/j.actbio.2010.03.014
Zhao Y-H, Chi Y-J. Characterization of collagen from eggshell membrane. Biotechnology. 2009;8:254-8. https://doi.org/10.3923/biotech.2009.254.258
Ahmed TA, Suso H-P, Hincke MT. In-depth comparative analysis of the chicken eggshell membrane proteome. J Proteomics. 2017;155:49-62. https://doi.org/10.1016/j.jprot.2017.01.002
Inoue N, Fukui A, Nomura M, Matsumoto M, Toyoshima K, Seya T. A novel chicken membrane-associated complement regulatory protein: Molecular cloning and functional characterization. J Immunol. 2001;166:424-31. https://doi.org/10.4049/jimmunol.166.1.424
Chato I, Rodríguez JÁ, Stoykova S, Chato J, Campos F, Carriel V. Comportamiento de fibroblastos humanos cultivados en las membranas de huevo para su aplicación en ingeniería tisular. Actual Médica. 2018;103:126-9. https://doi.org/10.15568/am.2018.805.or02
Guarderas F, Leavell Y, Sengupta T, Zhukova M, Megraw TL. Assessment of chicken-egg membrane as a dressing for wound healing. Adv Skin Wound Care. 2016;29:131-4. https://doi.org/10.1097/01.ASW.0000480359.58866.e9
Vuong TT, Rønning SB, Suso H-P, Schmidt R, Prydz K, Lundström M, et al. The extracellular matrix of eggshell displays anti-inflammatory activities through NF-κB in LPS-triggered human immune cells. J Inflamm Res. 2017;10:83-96. https://doi.org/10.2147/JIR.S130974
Yang J-Y, Chuang S-S, Yang W-G, Tsay P-K. Egg membrane as a new biological dressing in split-thickness skin graft donor sites: A preliminary clinical evaluation. Chang Gung Med J. 2003;26:153-9.
Zadik Y. Self-treatment of full-thickness traumatic lip laceration with chicken egg shell membrane. Wilderness Environ Med. 2007;18:230-1. https://doi.org/10.1580/06-WEME-LE-082R1.1
Ministerio de Medio Ambiente y Agua, Viceministerio de Agua Potable y Saneamiento Básico y Dirección General de Gestión Integral de Residuos Sólidos. Guía de Educación Ambiental en Gestión Integral de Residuos Sólidos. 1a edición. La Paz, Bolivia: MMAyA/VAPSB/DGGIRS; 2012.
Coca RD. Boletín Estadístico Anual de la Gestión 2017. Cochabamba: Asociación de Avicultores de Cochabamba (ADA-CBBA); 2018.
Vaclavik V, Christian EW. Essentials of food science. Third edition. New York, NY: Springer; 2008. p. 571.
Ministerio de Medio Ambiente y Agua. Diagnóstico de la gestión de residuos sólidos en Bolivia. 1a edición. La Paz, Bolivia: MMAyA/VAPSB/DGGIRS; 2011.
Hernández R, Fernández C, Baptista P. Metodología de la investigación. 6a edición. México, D.F.: McGraw-Hill; 2014.
Fuentes FM, Mendoza RA, Rosales AL, Cisneros RA. Instituto Nacional de Salud (Perú). Guía de manejo y cuidado de animales de laboratorio: ratón. Lima, Perú: Ministerio de Salud, Instituto Nacional de Salud; 2010. Fecha de consulta: 20 de febrero de 2020. Disponible en: https://repositorio.ins.gob.pe/handle/INS/117
Aragón J, Quintana Y, Aragón C, Hernández MJ. ImageJ: A free, easy, and reliable method to measure leg ulcers using digital pictures. Int J Low Extrem Wounds. 2017;16:269-73. https://doi.org/10.1177/1534734617744951
Siavash M, Shokri S, Haghighi S, Shahtalebi MA, Farajzadehgan Z. The efficacy of topical royal jelly on healing of diabetic foot ulcers: A double-blind placebo-controlled clinical trial. Int Wound J. 2013;12:137-42. https://doi.org/10.1111/iwj.12063
Jessup RL. What is the best method for assessing the rate of wound healing?: A comparison of 3 mathematical formulas. Adv Skin Wound Care. 2006;19:138-47.
https://doi.org/10.1097/00129334-200604000-00009
Quinn GP, Keough MJ. Experimental design and data analysis for biologists. Cambridge, UK, New York: Cambridge University Press; 2002. https://doi.org/10.1017/CBO9780511806384
Canadian Council on Animal Care. CCAC guidelines: Mice. Ottawa; 2019. Fecha de consulta: 20 de febrero de 2020. Disponible en: https://ccac.ca/Documents/Standards/Guidelines/CCAC_Guidelines_Mice.pdf
Tajima T, Kusamoto N. Fiber, fiber assembly, and fiber producing method. 2009 Fecha de consulta: 24 de marzo de 2020 . Disponible en: https://patents.google.com/patent/US20090031691A1/en?oq=US+2009%2f0031691+A1
Saqallah F, Hamed W, Talib W. In vivo evaluation of Antirrhinum majus’ wound-healing activity. Sci Pharm. 2018;86:45. https://doi.org/10.3390/scipharm86040045
Davidson JM, Yu F, Opalenik SR. Splinting strategies to overcome confounding wound contraction in experimental animal models. Adv Wound Care. 2013;2:142-8. https://doi.org/10.1089/wound.2012.0424
Vuong TT, Rønning SB, Ahmed TAE, Brathagen K, Høst V, Hincke MT, et al. Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes. PLoS ONE. 2018;13:e0201975. https://doi.org/10.1371/journal.pone.0201975
Glatz P, Miao Z, Rodda B. Handling and treatment of poultry hatchery waste: A review. Sustainability. 2011;3:216-37. https://doi.org/10.3390/su3010216
Ballester AM, Esquinas NC. Método de obtención de un producto cicatrizante y producto cicatrizante obtenido. WO2016055676A1, 2016. Fecha de consulta: 24 de marzo de 2020. Disponible en: https://patents.google.com/patent/WO2016055676A1/es?oq=WO+2016%2f055676+Al
Some similar items:
- Andrés F. Londoño, Silvana Levis, Juan D. Rodas, Hantavirus as important emerging agents in South America , Biomedica: Vol. 31 No. 3 (2011)
- Claudia Lucía Colorado, Guillermo Sánchez, Martha Inírida Guerrero, Clara Inés León, Reliability and agreement of two smear reading scales for classification and monitoring of multidrug therapy in leprosy patients , Biomedica: Vol. 31 No. 3 (2011)
- Jeison Monroy-Gómez, Orlando Torres-Fernández, Calbindin and parvalbumin distribution in spinal cord of normal and rabies-infected mice , Biomedica: Vol. 33 No. 4 (2013)
- Liliana María Zuluaga, John Camilo Hernández, Carlos Felipe Castaño, Jorge Hernando Donado, Effect of antenatal spiramycin treatment on the frequency of retinochoroiditis due to congenital toxoplasmosis in a Colombian cohort , Biomedica: Vol. 37 No. Sup.1 (2017): Suplemento 1, Alteraciones del sistema nervioso
- Anais Castellar, Marco Guevara, Juan D. Rodas, Andrés F. Londoño, Esteban Arroyave, Francisco J. Díaz, Silvana Levis, Pedro J. Blanco, First evidence of lymphocytic choriomeningitis virus (Arenavirus) infection in Mus musculus rodents captured in the urban area of the municipality of Sincelejo, Sucre, Colombia , Biomedica: Vol. 37 No. Sup.1 (2017): Suplemento 1, Alteraciones del sistema nervioso
- Marita Lardón, José Uberos, Eduardo Narbona, Does corticosteroid treatment during the pre and postnatal periods affect the neurodevelopmental outcome of premature newborns? , Biomedica: Vol. 37 No. Sup.1 (2017): Suplemento 1, Alteraciones del sistema nervioso
- Gerardo Santamaria, Jeison Monroy-Gómez, Orlando Torres-Fernández, Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice , Biomedica: Vol. 38 No. 2 (2018)
- Héctor Serrano-Coll, Olinto Mieles, Calixto Escorcia, Amparo Díaz, Camilo Beltrán, Nora Cardona-Castro, A case series of pure neural leprosy in patients diagnosed in a specialized center for the control of Hansen’s disease in Colombia , Biomedica: Vol. 38 No. 2 (2018)
- Fernando Ucan-Euan, Silvia Hernández-Betancourt, Madeleine Arjona-Torres, Alonso Panti-May, Marco Torres-Castro, Histopathological study in cardiac tissue of rodents infected with Trypanosoma cruzi, captured in suburbs of Mérida, México , Biomedica: Vol. 39 No. Supl. 2 (2019): Enfermedades transmisibles en el trópico, agosto
- Alejandra Mariel Canalis, Roberto Daniel Pérez, Gisele Evangelina Falchini, Elio Andrés Soria, Experimental acute arsenic toxicity in Balb/c mice: organic markers and splenic involvement , Biomedica: Vol. 41 No. 1 (2021)
