Prevalence of enterovirus infection in infants in Armenia, Colombia, 2009
Keywords:
Enterovirus, reverse transcriptase polymerase chain reaction, coxsackievirus infections, echovirus infections, feces.
Abstract
Introduction. Despite worldwide circulation of enteroviruses, little information has accumulated on the circulation of enteroviruses in Colombia.
Objective. The prevalence of enterovirus circulation was examined in children under 1 year to identify the most common enterovirus serotypes.
Materials and methods. Fecal samples were collected from 320 children under 1 year of age who attended a first-level health center in the city of Armenia, Colombia, in 2009. Enterovirus detection
was performed by reverse transcription reaction and nested polymerase chain reaction (RT-N-PCR) using generic enterovirus primers. Samples testing positive in the RT-N-PCR were inoculated into cell
cultures susceptible to enterovirus. All isolates were typed by seroneutralization with Lim-Benyesh-Melnick antiserum pools.
Results. Overall, enteroviral RNA was detected in 43 of 320 (13.3%; 95% CI: 9.7 to 17.1) fecal samples by RT-N-PCR. Viral isolation was possible in 26 of 43 (60.4%) of the positive samples. Of these, 15 were Coxsackievirus B (eight CVB1, two CVB2, five CVB5) and 11 Echovirus (six E6 and five E30).
Conclusions. The enteroviral circulation in a population on newly bornes up to 1 year old was 13.3%; the most frequent enterovirus was the same as those serotypes most commonly isolated in other arts
of the world. The use of RT-N-PCR was demonstrably feasible as a tool to monitor the presence of enterovirus in stool samples.
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References
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16. Trallero G, Avellon A, Otero A, De Miguel T, Pérez C, Rabella N, et al. Enteroviruses in Spain over the decade 1998-2007: Virological and epidemiological studies. J Clin Virol. 2010;47:170-6.
17. Savolainen-Kopra C, Paananen A, Blomqvist S, Klemola P, Simonen ML, Lappalainen M, et al. A large Finnish echovirus 30 outbreak was preceded by silent circulation of the same genotype. Virus Genes. 2011;42:28-36.
18. Tan CY, Ninove L, Gaudart J, Nougairede A, Zandotti C, Thirion-Perrier L, et al. A retrospective overview of enterovirus infection diagnosis and molecular epidemiology in the public hospitals of Marseille, France (1985-2005). PLoS One. 2011;6:e18022.
19. Farías A, Cabrerizo M, Ré V, Glatstein N, Pisano B, Spinsanti L, et al. Molecular identification of human enteroviruses in children with neurological infections from the central region of Argentina. Arch Virol. 2011;156:129-33.
20. Pinto Junior VL, Rebelo MC, Costa EV, Silva EE, Bóia MN. Description of a widespread outbreak of aseptic meningitis due to echovirus 30 in Rio de Janeiro state, Brazil. Braz J Infect Dis. 2009;13:367-70.
21. Aguirre C, Vallejo I, Vargas A, Acevedo L, Uribe G, Londoño A. Epidemia de meningitis viral en Medellín, Colombia, 2004. Revista de Salud Pública Medellín. 2006;1:67-82.
22. Santos AP. Echovirus 6 associated with exanthematic disease. Rev Soc Bras Med Trop. 2008;41:672-5.
23. Mao N, Zhao L, Zhu Z, Chen X, Zhou S, Zhang Y, et al. An aseptic meningitis outbreak caused by echovirus 6 in Anhui province, China. J Med Virol. 2010;82:441-5.
24. Yen FB, Chang LY, Kao CL, Lee PI, Chen CM, Lee CY. Coxsackieviruses infection in northern Taiwan-epidemiology and clinical characteristics. J Microbiol Immunol Infect. 2009;42:38-46.
25. Andréoletti L, Ventéo L, Douche-Aourik F, Canas F, Lorin D, Grandmaison G, et al. Active Coxsackieviral B infection is associated with disruption of dystrophin in endomyocardial tissue of patients who died suddenly of acute myocardial infarction. Am Coll Cardiol. 2007;50:2207-14.
26. Peláez D, Boshell J, Oberste S, Pallansh A, Brown B. Enterovirus no polio de casos con parálisis residual en Colombia, 1992-1995. Biomédica. 1999;2:144-58.
27. Bello M, Mas P, Palomera R, Morier L, Avalos I, Acosta B et al. Meningoencefalitis virales por enterovirus en el período 1990-1995. Rev Arg Microbiol .1997;29:176-83.
28. Más P, Cáceres V, Galindo M, Gary H, Valcárcel M, Barrios J, et al. Persistence of vaccine-derived Poliovirus following a mass vaccination campaign in Cuba: Implications for stopping polio vaccination after global eradication. Inter J Epidemiol. 2001;30:1029-34.
2. Tebruegge M, Curtis N. Enterovirus infections in neonates. Semin Fetal Neonatal Med. 2009;4:222-7.
3. Romero JR. Pediatric group B coxsackievirus infections. Curr Top Microbiol Immunol. 2008;323:223-39.
4. Oberste M, Pallansch MA. Establishing evidence for enterovirus infection in chronic disease. Ann NY Acad Sci.2003;1005:23-31.
5. Khetsuriani N, LaMonte-Fowlkes A, Oberste MS, Pallansch MA. Enterovirus surveillance-United States, 1970-2005. MMWR Surveill Summ. 2006;55:1-20.
6. Sarmiento L. Enteroviral meningitis and emergence of rare enterovirus types: Cuban experience. In: Strong PV, editor. Focus on meningitis research. New York: Nova Science Publishers, Inc; 2004. p. 1-14.
7. Sarmiento L, Mas P, Goyenechea A, Palomera R, Morier L, Capo V, et al. First epidemic of echovirus 16 meningitis in Cuba. Emerg Infect Dis. 2001;7:887-9.
8. Kitchin P, Bootman Y. Quality control of polymerase chain reaction. Rev Med Virol. 1993;3:107-14.
9. American Type Culture Collection. Catalogue of cell lines and hybridomas. 7th ed. Rockville: American Type Culture Collection. 1992. p. 48.
10. Melnick J. Enteroviruses: Polioviruses, coxsackieviruses, echoviruses and newer enterovirus. In: Fields B, Knipe D, Howley P, editors. Field's virology. 3rd ed. Philadelphia, Pa:
Lippincott Raven Publisher; 1996. p. 655-712.
11. Melnick JL, Wimber IL. Liophilized combination of the Enterovirus equine antisera. New LBM pools prepared from reserves of antisera stored frozen for two decades. Bull World Health Organ. 1984;63:543-50.
12. Centers for Disease Control and Prevention (CDC). Nonpolio enterovirus and human parechovirus surveillance - United States, 2006-2008. MMWR Morb Mortal Wkly Rep. 2010;59:1577-80.
13. Strikas R, Anderson L, Parker R. Temporal and geographic patterns of isolates of nonpolio enterovirus in the United States, 1970-1983. J Infect Dis 1986;153:346-51.
14. Centers for Disease Control and Prevention. Nonpolio enterovirus surveillance-United States, 1993-1996. MMWR Morb Mortal Wkly Rep. 1997;46:748-50.
15. CosiÄ G, DuriÄ P, MiloseviÄ V, DekiÄ J, Canak G, Turkulov V. Ongoing outbreak of aseptic meningitis associated with echovirus type 30 in the City of Novi Sad, Autonomous Province of Vojvodina, Serbia, June-July 2010. Euro Surveill. 2010;15:19638.
16. Trallero G, Avellon A, Otero A, De Miguel T, Pérez C, Rabella N, et al. Enteroviruses in Spain over the decade 1998-2007: Virological and epidemiological studies. J Clin Virol. 2010;47:170-6.
17. Savolainen-Kopra C, Paananen A, Blomqvist S, Klemola P, Simonen ML, Lappalainen M, et al. A large Finnish echovirus 30 outbreak was preceded by silent circulation of the same genotype. Virus Genes. 2011;42:28-36.
18. Tan CY, Ninove L, Gaudart J, Nougairede A, Zandotti C, Thirion-Perrier L, et al. A retrospective overview of enterovirus infection diagnosis and molecular epidemiology in the public hospitals of Marseille, France (1985-2005). PLoS One. 2011;6:e18022.
19. Farías A, Cabrerizo M, Ré V, Glatstein N, Pisano B, Spinsanti L, et al. Molecular identification of human enteroviruses in children with neurological infections from the central region of Argentina. Arch Virol. 2011;156:129-33.
20. Pinto Junior VL, Rebelo MC, Costa EV, Silva EE, Bóia MN. Description of a widespread outbreak of aseptic meningitis due to echovirus 30 in Rio de Janeiro state, Brazil. Braz J Infect Dis. 2009;13:367-70.
21. Aguirre C, Vallejo I, Vargas A, Acevedo L, Uribe G, Londoño A. Epidemia de meningitis viral en Medellín, Colombia, 2004. Revista de Salud Pública Medellín. 2006;1:67-82.
22. Santos AP. Echovirus 6 associated with exanthematic disease. Rev Soc Bras Med Trop. 2008;41:672-5.
23. Mao N, Zhao L, Zhu Z, Chen X, Zhou S, Zhang Y, et al. An aseptic meningitis outbreak caused by echovirus 6 in Anhui province, China. J Med Virol. 2010;82:441-5.
24. Yen FB, Chang LY, Kao CL, Lee PI, Chen CM, Lee CY. Coxsackieviruses infection in northern Taiwan-epidemiology and clinical characteristics. J Microbiol Immunol Infect. 2009;42:38-46.
25. Andréoletti L, Ventéo L, Douche-Aourik F, Canas F, Lorin D, Grandmaison G, et al. Active Coxsackieviral B infection is associated with disruption of dystrophin in endomyocardial tissue of patients who died suddenly of acute myocardial infarction. Am Coll Cardiol. 2007;50:2207-14.
26. Peláez D, Boshell J, Oberste S, Pallansh A, Brown B. Enterovirus no polio de casos con parálisis residual en Colombia, 1992-1995. Biomédica. 1999;2:144-58.
27. Bello M, Mas P, Palomera R, Morier L, Avalos I, Acosta B et al. Meningoencefalitis virales por enterovirus en el período 1990-1995. Rev Arg Microbiol .1997;29:176-83.
28. Más P, Cáceres V, Galindo M, Gary H, Valcárcel M, Barrios J, et al. Persistence of vaccine-derived Poliovirus following a mass vaccination campaign in Cuba: Implications for stopping polio vaccination after global eradication. Inter J Epidemiol. 2001;30:1029-34.
How to Cite
1.
González MM, Giraldo AM, Quintero L, Padilla L, Sarmiento L, Castaño JC. Prevalence of enterovirus infection in infants in Armenia, Colombia, 2009. Biomed. [Internet]. 2011 Jun. 30 [cited 2025 Apr. 5];31(4):545-51. Available from: https://revistabiomedicaorg.biteca.online/index.php/biomedica/article/view/424
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