Novel larvicide tablets of Bacillus thuringiensis var. israelensis: Assessment of larvicidal effect on Aedes aegypti (Diptera: Culicidae) in Colombia
Abstract
Introduction: Aedes (Stegomyia) aegypti is the vector for dengue, chikungunya, and Zika arboviruses. Bti-CECIF is a bioinsecticide designed and developed in the form of a solid tablet for the control of this vector. It contains Bacillus thuringiensis var. israelensis (Bti) serotype H-14.
Objective: To evaluate under semi-field and field conditions the efficacy and residual activity of Bti-CECIF tablets on Aedes aegypti larvae in two Colombian municipalities.
Materials and methods: We tested under semi-field conditions in plastic tanks (Rotoplast™) four different Bti doses (0.13, 0.40, 0.66 and 0.93 mg/L) in the municipality of Apartadó, department of Antioquia, to assess Bti-CECIF efficacy (percentage of reduction of larval density) and the residual activity in water tanks containing A. aegypti third-instar larvae. The efficacy and residuality of the most lethal dose were subsequently evaluated under field conditions in cement tanks in the municipality of San Carlos, department of Córdoba.
Results: Under semi-field conditions, the highest tested dose exhibited the greatest residual activity (15 days) after which larval mortality was 80%. Under field conditions, the highest tested Bti-CECIF doses showed 100% mortality and exhibited a residual activity of seven days in 90% of the tanks.
Conclusion: Bti-CECIF tablets effectively controlled A. aegypti larvae under field conditions for up to seven days post-treatment.
Downloads
References
Musgrove P, Creese A, Preker A, Baeza C, Anell A, Prentice T. Health Systems: Improving perfomance. Geneva: World Health Organization; 2000.
Gürtler RE, Garelli FM, Coto HD. Effects of a five-year citywide intervention program to control Aedes aegypti and prevent dengue outbreaks in northern Argentina. PLoS Negl Trop Dis. 2009;3:e427. https://doi.org//10.1371/journal.pntd.000042
Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013;496:504-7. https://doi.org//10.1038/nature12060
Organización Panamerica de la Salud/Organización Mundial de la Salud. Casos reportados de dengue en las Américas, 2017. Accessed: March 5, 2018. Available from:
http://www.paho.org/data/index.php/es/temas/indicadoresdengue/dengue-nacional/9-dengue-pais-ano.html
Instituto Nacional de Salud de Colombia. Informe del evento dengue, Colombia, 2013. Accessed: March 5, 2018. Available from: http://www.ins.gov.co/buscador-eventos/Informesdeevento/Dengue 2013.pdf
Instituto Nacional de Salud. Informe del evento dengue, Colombia, 2014. Accessed: March 5, 2018. Available from: http://www.ins.gov.co/buscador-eventos/Informesdeevento/Dengue 2014.pdf
Instituto Nacional de Salud. Informe del evento dengue, Colombia, 2015. Accessed: March 5, 2018. Available from: http://www.ins.gov.co/buscador-eventos/Informesdeevento/Dengue 2015.pdf
Instituto Nacional de Salud. Informe del evento de dengue, Colombia, 2016. Accessed: March 5, 2018. Available from: http://www.ins.gov.co/buscador-eventos/Informesdeevento/Dengue 2016.pdf
Instituto Nacional de Salud. Informe del evento dengue, Colombia, 2017. Accessed: March 5, 2018. Available from: http://www.ins.gov.co/buscador-eventos/Informesdeevento/DENGUEPEXIII2017.pdf
Instituto Nacional de Salud. Informe del evento chikungunya, Colombia, 2015. Accessed: March 5, 2018. Available from: http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/Informe de Evento Epidemiolgico/CHIKUNGUNYAPeriodo XIII 2015.pdf
Instituto Nacional de Salud. Informe del evento chikungunya, Colombia, 2016. Accessed: March 5, 2018. Available from: http://www.ins.gov.co/buscador-eventos/Informesdeevento/Chikunguña 2016.pdf
Instituto Nacional de Salud. Informe del evento Zika, Colombia - semanas epidemiológicas 32 de 2015 a 52 de 2016. Accessed: March 5, 2018. Available from: http://www.ins.gov.co/Noticias/ZIKA/Casos zika por municipio semana 52 2016.pdf
World Health Organization. Dengue, 2018. Accessed: March 5, 2018. Available from: http://www.who.int/mediacentre/factsheets/fs117/es/
Organización Panamerica de la Salud/Organización Mundial de la Salud. Resolución CD55.R6: Estrategia para la prevención y control de las enfermedades arbovirales, 2016. Accessed: March 5, 2018. Available from: https://www.paho.org/hq/index.php?option=com_docman&task=doc
Becker N, Zgomba M, Ludwig M, Petric D, Rettich F. Factors influencing the activity of Bacillus thuringiensis var. israelensis treatments. J Am Mosq Control Assoc. 1992;8:285-9.
De Barjac H. Étude cytologique de l’action de Bacillus thuringiensis var. israelensis sur larves de moustiques. Comptes Rendus de l’Académie des Sci. 1978;286:1629-32.
Ponce G. Efecto de concentraciones subletales de Bacillus thuringiensis israelensis H-14 Vectobac AS® en parámetros biológicos de Ae. aegypti (tesis). San Nicolás de los Garza, N.L., México: Universidad Autónoma de Nuevo León; 1999.
Suárez M, Ayala W, Nelson M. Evaluacion de dos formulaciones de Bacillus thuringiensis H-14 para el control de larvas de Aedes aegypti. Biomédica. 1987;7:5-12. https://doi.org/10.7705/biomedica.v7i1-2.1928
Gerberg E. Manual for mosquito rearing and experimental techniques. Am Mosq Control Assoc. 1970;5:1-9.
World Health Organization. Guidelines for laboratory and field testing of mosquito larvicides. Geneva: WHO; 2005.
Cadavid JM. Estructura genética microgeográfica y susceptibilidad a insecticidas de Aedes aegypti de Medellín. Medellín: Universidad de Antioquia; 2016. p. 79.
Departamento Administrativo Nacional de Estadística. Documento: Esquema de Ordenamiento Territorial. Municipio de San Carlos, Córdoba: DANE; 2005.
Mulla MS, Norland LR, Fanara DM, Darwazeh HA, McKean DW. Control of chironomid midges in recreational lakes. J Econ Entomol. 1971;64:300-7.
Ocampo CB, González C, Morales CA, Pérez M, Wesson D, Apperson CS. Evaluation of community-based strategies for Aedes aegypti control inside houses. Biomédica. 2009;29:282-97. https://doi.org//S0120-41572009000200020
Marcombe S, Darriet F, Agnew P, Etienne M, Yp-Tcha MM, Yébakima A, et al. Field efficacy of new larvicide products for control of multi-resistant Aedes aegypti populations in Martinique (French West Indies). Am J Trop Med Hyg. 2011;84:118-26.
World Health Organization. Bacillus thuringiensis israelensis (Bti) in drinking-water. Geneva: World Health Organization; 2009. p. 8.
Instituto Nacional de Salud. Informe epidemiológico: Red de vigilancia de la resistencia a insecticidas de uso en salud pública en Colombia 2004-2014. Bogotá: Instituto Nacional de Salud; 2014. p. 27.
Maestre R, Rey G, de las Salas J, Vergara C, Santacoloma L, Goenaga S, et al. Estado de la susceptibilidad de Aedes aegypti a insecticidas en Atlántico (Colombia). Rev Colomb Entomol. 2010;36:242-8.
Conde M, Orjuela LI, Castellanos CA, Herrera-Varela M, Licastro S, Quiñones ML. Evaluación de la sensibilidad a insecticidas en poblaciones de Aedes aegypti (Diptera: Culicidae) del departamento de Caldas, Colombia, en 2007 y 2011. Biomédica. 2014;35:43-52. https://doi.org//10.7705/biomedica.v35i1.23
Maestre R, Rey G, de las Salas J, Vergara C, Santacoloma L, Goenaga S, et al. Susceptibilidad de Aedes aegypti (Diptera: Culicidae) a temefos en Atlántico-Colombia. Rev Colomb Entomol. 2009;35:202-5.
Vilarinhos P, Monnerat R. Larvicidal persistence of formulations of Bacillus thuringiensis var. israelensis to control larval Aedes aegypti. J Am Mosq Control Assoc. 2004;20:311-4.
Zequi JAC, Lopes J, Santos FP. Efficacy and persistence of two Bacillus thuringiensis israelensis formulations for the control of Aedes aegypti (Linnaeus, 1762) under simulated field conditions. Int J Mosq Res. 2015;2:5-9.
Melo-Santos MAV de, Gomes Sanchez E, de Jesus FJ, Regis L. Evaluation of a new tablet formulation based on Bacillus thuringiensis sorovar. israelensis for larvicidal control of Aedes aegypti. Mem Inst Oswaldo Cruz. 2001;96:859-60.
Boyce R, Lenhart A, Kroeger A, Velayudhan R, Roberts B, Horstick O. Bacillus thuringiensis israelensis (Bti) for the control of dengue vectors: Systematic literature review. Trop Med Int Heal. 2013;18:564-577. https://doi.org//10.1111/tmi.12087
Becker N, Djakaria S, Kaiser A, Zulhasril O, Ludwig HW. Efficacy of a new tablet formulation of an asporogenous strain of Bacillus thuringiensis israelensis against larvae of Aedes aegypti. Bull Soc Vector Ecol. 1991;16:176-82.
Armengol G, Hernández J, Vélez JG, Orduz S. Longlasting effects of a Bacillus thuringiensis serovar israelensis experimental tablet formulation for Aedes aegypti (Diptera: Culicidae) control. J Econ Entomol. 2006;99:1590-1595. https://doi.org//10.1093/jee/99.5.1590
Mahilum MM, Ludwig M, Madon MB, Becker N. Evaluation of the present dengue situation and control strategies against Aedes aegypti in Cebu City, Philippines. J vector Ecol. 2005;30:277-83.
Mulla MS, Thavara U, Tawatsin A, Chompoosri J. Procedures for evaluation of field efficacy of slow-release formulations of larvicides against Aedes aegypti in waterstorage containers. J Am Mosq Control Assoc. 2004;20:64-73.
Benjamin S, Rath A, Chiang YF, Lee HL. Efficacy of a Bacillus thuringiensis israelensis tablet formulation, VectoBac DT®, for control of dengue mosquito vectors in potable water containers. Southeast Asian J Trop Med Public Health. 2005;36:879-92.
Monnerat R, Dumas V, Ramos F, Pimentel L, Nunes A, Sujii E, et al. Evaluation of different larvicides for the control of Aedes aegypti (Linnaeus) (Diptera: Culicidae) under simulated field conditions. BioAssay. 2012;7:1-4.
Tetreau G, Stalinski R, Kersusan D, Veyrenc S, David JP, Reynaud S, et al. Decreased toxicity of Bacillus thuringiensis subsp. israelensis to mosquito larvae after contact with leaf litter. Appl Environ Microbiol. 2012;78:5189-95. https://doi.org//10.1128/AEM.00903-12
Ben-Dov E, Saxena D, Wang Q, Manasherob R, Boussiba S, Zaritsky A. Ingested particles reduce susceptibility of insect larvae to Bacillus thuringiensis. J Appl Entomol. 2003;127:146-52. https://doi.org//10.1046/j.1439–0418.2003
Kroeger A, Dehlinger U, Burkhardt G, Atehortua W, Anaya H, Becker N. Community based dengue control in Columbia: People’s knowledge and practice and the potential contribution of the biological larvicide Bti (Bacillus thuringiensis israelensis). Trop Med Parasitol. 1995;46:241-6.
Phan-Urai P, Kong-ngamsuk W, Malainual N. Field trial of Bacillus thuringiensis H-14 (Larvitab®) against Aedes aegypti larvae in Amphoe Khlung, Chanthaburi Province, Thailand. J Trop Med Parasitol. 1995;18:106-14.
Batra CP, Mittal PK, Adak T. Control of Aedes aegypti breeding in desert coolers and tires by use of Bacillus thuringiensis var. israelensis formulation. J Am Mosq Control Assoc. 2000;16:321-3.
Setha T, Chantha N, Socheat D, Benjamin S. Efficacy of VectoBac WG, a Bacillus thuringiensis israelensis formulation, to control dengue mosquito vectors in Cambodia. Am J Trop Med Hyg. 2007;77:256-7.
Ortegón-Cárdenas L, Carvajal-López D. Efectividad del Bacillus thuringiensis israelensis como control biológico para larvas de Aedes aegypti en Florencia (Caquetá, Colombia). Momentos Cienc. 2009;6:100-4.
Ponce G, Flores AE, Badii MH, Fernández I, Rodríguez ML. Evaluación de Bacillus thuringiensis isaraelensis (VectoBac 12 ASâ ) sobre la población larval de Aedes aegypti en el área metropolitana de Monterrey N. L. México. Rev la Fac Salud Pública y Nutr Univ Autónoma León. 2003;4:3-9.
Organización Panamericana de la Salud/Organización Mundial de la Salud. La estrategia de gestión integrada para la prevención y el control del dengue en la Región de las Américas. Washington, D.C.: OPS/OMS; 2017. p. 70.
Some similar items:
- Jorge L. Alvarado-Socarras, Fredi Alexander Díaz-Quijano, Alfonso J. Rodríguez-Morales, Letters to editor , Biomedica: Vol. 36 (2016): Suplemento 2, Enfermedades virales
- José Joaquín Carvajal, Nildimar Alves Honorio, Silvia Patricia Díaz, Edinso Rafael Ruiz, Jimmy Asprilla, Susanne Ardila, Gabriel Parra-Henao, Detection of Aedes albopictus (Skuse) (Diptera: Culicidae) in the municipality of Istmina, Chocó, Colombia , Biomedica: Vol. 36 No. 3 (2016)
- Marcela Conde, Lorena I. Orjuela, Cesar Augusto Castellanos, Manuela Herrera-Varela, Susana Licastro, Martha L. Quiñones, Insecticide susceptibility evaluation in Aedes aegypti populations of Caldas, Colombia, in 2007 and 2011 , Biomedica: Vol. 35 No. 1 (2015)
- María Elena Cuéllar-Jiménez, Olga Lucía Velásquez-Escobar, Ranulfo González-Obando, Carlos Andrés Morales-Reichmann, Detection of Aedes albopictus (Skuse) (Diptera: Culicidae) in the city of Cali, Valle del Cauca, Colombia , Biomedica: Vol. 27 No. 2 (2007)
- Daniel Elías Cuartas, Genny Martínez, Diana María Caicedo, Jhonny Garcés, Yoseth Ariza-Araujo, Miguel Peña, Fabián Mendéz, Spatial distribution of potential and positive Aedes aegypti breeding sites , Biomedica: Vol. 37 No. Sup. 2 (2017): Suplemento 2, Entomología médica, 2017
- Elizabeth Borrero, Gabriel Carrasquilla, Neal Alexander, Decentralization and health system reform: What is their impact on malaria incidence in Colombian municipalities? , Biomedica: Vol. 32 (2012): Suplemento 1, Malaria
- José Joaquín Carvajal, Ligia Inés Moncada, Mauricio Humberto Rodríguez, Ligia del Pilar Pérez, Víctor Alberto Olano, Characterization of Aedes albopictus (Skuse, 1894) (Diptera:Culicidae) larval habitats near the Amazon River in Colombia , Biomedica: Vol. 29 No. 3 (2009)
- Sandy Milena Caldera, María Cristina Jaramillo, Suljey Cochero, Alveiro Pérez-Doria, Eduar Elías Bejarano, Genetic differences between populations of Aedes aegypti from municipalities in northern Colombia, with high and low dengue incidence , Biomedica: Vol. 33 (2013): Suplemento 1, Fiebres hemorrágicas
- Érika Patricia Alarcón, Ángela María Segura, Guillermo Rúa-Uribe, Gabriel Parra-Henao, Ovitraps evaluation for surveillance and control of Aedes aegypti in two urban settlements of Urabá, Antioquia , Biomedica: Vol. 34 No. 3 (2014)
- Jorge R. Rey, Philip Lounibos, Ecology of Aedes aegypti and Aedes albopictus in the Americas and disease transmission , Biomedica: Vol. 35 No. 2 (2015)
| Article metrics | |
|---|---|
| Abstract views | |
| Galley vies | |
| PDF Views | |
| HTML views | |
| Other views | |
