Characterization of clinical isolates of Mycobacterium tuberculosis from HIV positive individuals in Colombia, 2012
Abstract
Introduction: One third of the increase in tuberculosis cases is attributed to the spread of HIV. In 2012, 1,397 HIV-associated tuberculosis cases were reported in Colombia, i.e., 11.8% of the total cases. Molecular epidemiology tools help to understand the transmission of tuberculosis.
Objective: To characterize clinical isolates of Mycobacterium tuberculosis derived from HIV-infected individuals, received at the Laboratorio Nacional de Referencia in the Instituto Nacional de Salud.
Materials and methods: This was a descriptive observational study. We analyzed 63 isolates of M. tuberculosis from HIV-infected individuals. Identification, drug susceptibility and genotyping assays were performed.
Results: Of the new cases evaluated, three (5.0%) were resistant to isoniazid combined with streptomycin; two (3.3%) to rifampicin, and one (1.6%) to isoniazid. Previously treated cases were sensitive. No multidrug resistance was evident. Among the predominant genotypes, 20 isolates were (31.7%) LAM9, eight (12.7%), H1, and seven (11.1%), T1. Nineteen isolates corresponded to orphan patterns. One single grouping was observed among tested isolates. We found no statistically significant
difference between the proportions of the antituberculous drug resistance and genotypes.
Conclusion: We found resistant isolates to the most powerful drugs, rifampicin and isoniazid, among new cases, showing the transmission of resistant strains. Genetic families of M. tuberculosis LAM9, T1 and H1 correspond to those described in the general population. We detected no active transmission among studied isolates. More comprehensive studies are needed to assess the real situation of HIV associated tuberculosis in the country regarding sensitivity and transmission.
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References
World Health Organization. Global tuberculosis control: WHO report 2013. Geneva: WHO; 2013.
Organización Panamericana de la Salud. Coinfección TB-HIV. Guía clínica. Washington, D.C.: OPS; 2008.
Instituto Nacional de Salud. Informe del evento tuberculosis hasta el periodo epidemiológico 13 del año 2012. Fecha de consulta: 28 de julio de 2014. Disponible
Ministerio de la Protección Social. Circular externa 058 de 2009. Lineamientos para el manejo programático de tuberculosis y lepra en Colombia. Bogotá, D.C.: Ministerio de la Protección Social; 2009.
Fenner L, Egger M, Bodmer T, Furrer H, Ballif M, Battegay M, et al. HIV infection disrupts the sympatric host–pathogen relationship in human tuberculosis. PLoS Genet. 2013;9:e1003318. http//dx.doi.org/10.1371/journal.pgen.1003318
Adams LV, Kreiswirth B, Albert R, Soini H, Mtel L, Matee M, et al. Molecular epidemiology of HIV-associated tuberculosis in Dar es Salaam, Tanzania: Strain predominance, clustering and polyclonal disease. J Clin Microbiol. 2013;50:2645-50.
http//dx.doi.org/10.1128/JCM.00624-12
Úriz J, Repáraz J, Castiello J, Sola J. Tuberculosis en pacientes infectados por el VIH. An Sist Sanit Navar. 2007;30(Suppl.2):S131-42.
Bruchfeld J, Correia-Neves M, Källenius G. Tuberculosis and HIV coinfection. Cold Spring Harb Perspect Med. 2015;5:a017871. http//dx.doi.org/10.1101/cshperspect.a017871
Houben RM, Glynn JR, Mallard K, Sichali L, Malema S, Fine PE, et al. Human immunodeficiency virus increases the risk of tuberculosis due to recent re-infection in individuals with latent infection. Int J Tuberc Lung Dis. 2010;14:909-15.
Middelkoop K, Mathema B, Myer L, Shashkina E, Whitelaw A, Gilla Kaplan G, et al. Transmission of tuberculosis in a South African community with a high prevalence of HIV infection. J Infect Dis. 2015;211:53-61. http//dx.doi.org/10.1093/infdis/jiu403
Organización Mundial de la Salud. Cuestiones prioritarias en materia de investigación sobre la tuberculosis y la infección por el VIH en entornos de recursos limitados donde la infección por el VIH es prevalente. Ginebra: OMS; 2011.
Instituto Nacional de Salud. Diagnóstico bacteriológico de tuberculosis y micobacteriosis. Bogotá, D.C.: INS; 2012.
Becton Dickinson. Manual técnico de MGIT 960® para evaluación de resistencia a fármacos antituberculosos.
Heidelberg: Becton Dickinson; 2009.
Springer B, Lucke K, Calligaris-Maibach R, Ritter C, Böttger E. Quantitative drug susceptibility testing of Mycobacterium tuberculosis by use of MGIT 960 and EpiCenter instrumentation. J Clin Microbiol. 2009;47:1773-80. http//dx.doi.org/10.1128/JCM.02501-08
Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997;35: 907-14.
Asencios L, Vásquez L, Leo E, Quispe N, Huaroto L, Cabezas C. Niveles de resistencia a drogas antituberculosas en pacientes con coinfección VIH/tuberculosis, Lima, 1998-2001. Rev Per Med Exper Salud Pública. 2006;23:98-103. http://dx.doi.org/10.17843/rpmesp.2006.232.1068
De Paz D, Potes L, Quiñónez E, Martínez L, Cepeda M, Guarín N, et al. Resistencia a fármacos antituberculosis en pacientes coinfectados con tuberculosis y virus de la inmunodeficiencia humana, en un hospital de referencia de 2007 a 2010 en Cali (Colombia). Infectio. 2013;16:161-5.
González J, Estrada S, Álvarez H. Resistencia primaria a los medicamentos anti tuberculosos en pacientes VIH positivos y pacientes VIH negativos con tuberculosis en Medellín, Colombia. Infectio. 2001;5:223-34.
Realpe T, Correa N, Rozo JC, Ferro BE, Gómez V, Zapata E, et al. Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia. PLoS ONE. 2014;9:e93848. http//dx.doi.org/10. 1371/journal.pone.0093848
Puerto G, Erazo L, Wintaco M, Castro C, Ribón W, Guerrero M. Mycobacterium tuberculosis genotypes determined by spoligotyping to be circulating in Colombia between 1999 and 2012 and their possible associations with transmission and susceptibility to first-line drugs. PLoS ONE. 2015;10:e0124308. http//dx.doi.org/10.1371/journal.pone.0124308
Cerezo I, Jiménez Y, Hernández J, Zozio T, Murcia M, Rastogi N. A first insight on the population structure of Mycobacterium tuberculosis complex as studied by spoligotyping and MIRU-VNTRs in Bogotá, Colombia. Infect Genet Evol. 2012;12:657-63. http//dx.doi.org/10.1016/j.meegid.2011.07.006
Rosales S, Pineda-García L, Ghebremichael S, Rastogi N, Hoffner S. Molecular diversity of Mycobacterium tuberculosis isolates from patients with tuberculosis in Honduras. BMC Microbiol. 2010;10:208. http//dx.doi.org/10.1186/1471-2180-10-208
Gonzalo X, Ambroggi M, Córdova E, Brown T, Poggi S, Drobniewski F. Molecular epidemiology of Mycobacterium tuberculosis, Buenos Aires, Argentina. Emerg Infect Dis.
;17:528-31. http//dx.doi.org/10.3201/eid1703.100394
Taype CA, Agapito JC, Accinelli RA, Espinoza JR, Godreuil S, Goodman SJ, et al. Genetic diversity, population structure and drug resistance of Mycobacterium tuberculosis in Perú. Infect Genet Evol. 2012;12:577-85. http//dx.doi.org/10.1016/j.meegid.2012.02.002
Hernández J, Murcia M, de la Hoz H. Epidemiología molecular de la tuberculosis en Bogotá en aislamientos clínicos obtenidos durante 11 años. Rev Salud Pública. 2008;10:126-36. http://dx.doi.org/10.1590/S0124-00642008000100012
Liu J, Wang HZ, Lian LL, Yu YH, Zhao XQ, Guo CP, et al. Molecular characteristics and drug susceptibility of Mycobacterium tuberculosis isolates from patients coinfected with human immunodeficiency virus in Beijing, China. Biomed Environ Sci. 2015;28:222-6. http://dx.doi.org/10.3967/bes2015.030
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