Coinfection of hepatitis E virus and other hepatitis virus in Colombia and its genotypic characterization
Abstract
Introduction: Hepatitis E virus has emerged as a public health problem, particularly in developing countries. The four genotypes identified in mammals include the G3 found in indigenous hepatitis in countries and regions with high porcine population, and the G1, associated with maternal deaths. Objective: To determine coinfection by hepatitis E virus and the circulating genotypes in Colombia in 1,097 samples using serological markers for hepatitis A, B and C. Materials and methods: Serum samples of 1,097 patients from different regions of Colombia stored at the Laboratorio de Virología of the Instituto Nacional de Salud were selected to detect IgG and IgM anti-hepatitis E virus antibodies. The viral genomes of positive samples were amplified by RT-PCR, and the products were sequenced and phylogenetically analyzed by comparing ORF2 sequences deposited in the GenBank. Results: IgG anti-hepatitis E virus antibodies were found in 278 samples, IgM in 62, and both markers in 64. Hepatitis E virus and hepatitis A virus coinfection determined by IgG anti-hepatitis E virus was 33.6% and 16.1% by IgM; hepatitis E virus and hepatitis B virus coinfection was 23.4% and 8.1%, and hepatitis E virus and hepatitis C virus coinfection was 35.4% and 5.83%, respectively. Among the 52 positive samples by PCR nine were sequenced and grouped within genotype 3A of the American porcine strain. Conclusions: The highest seropositivity was observed for hepatitis A and E. The incidence of hepatitis E virus coinfection with other hepatotropic viruses indicated that this pathogen is more frequent than expected. The circulation of genotype 3A implies that this disease may occur in outbreaks and as zoonosis in Colombia.
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Pérez-Gracia MT, Suay B, Mateos-Lindemann ML. Hepatitis E: An emerging disease. Infect Genet Evol. 2014;22:40-59. http://dx.doi.org/10.1016/j.meegid.2014. 01.002
Dalton HR, Bendall R, Ijaz S, Banks M. Hepatitis E: An emerging infection in developed countries. Lancet Infect Dis. 2008;8:698-709. http://dx.doi.org/10.1016/S1473-3099 (08)70255-X
Navaneethan U, Al Mohajer M, Shata MT. Hepatitis E and pregnancy: Understanding the pathogenesis. Liver Int. 2008;28:1190-9. http://dx.doi.org/10.1111/j.1478-3231.2008. 01840.x
Smith DB, Simmonds P, Jameel S, Emerson SU, Harrison TJ, Meng XJ, et al. Consensus proposals for classification of the family Hepeviridae. J Gen Virol. 2014;95:2223-32. http://dx.doi.org/10.1099/vir.0.068429-0
Purdy MA, Khudyakov YE. Evolutionary history and population dynamics of hepatitis e virus. PLoS One. 2010;5:e14376. http://dx.doi.org/10.1371/journal.pone. 0014376
Tam AW, Smith MM, Guerra ME, Huang CC, Bradley DW, Fry KE, et al. Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome. Virology. 1991;185:120-31. http://dx.doi.org/10.1016/0042-6822(91)90760-9
Echevarría JM, González JE, Lewis-Ximénez LL, Lopes dos Santos DR, Munne MS, Pinto MA, et al. Hepatitis E virus infection in Latin America: A review. J Med Virol. 2013;85:1037-45. http://dx.doi.org/10.1002/jmv.23526
Buisson Y, Grandadam M, Nicand E, Cheval P, van Cuyck-Gandre H, Innis B, et al. Identification of a novel hepatitis E virus in Nigeria. J Gen Virol. 2000;81:903-9. http://dx.doi.org/10.1099/0022-1317-81-4-903
van Cuyck-Gandre H, Zhang HY, Tsarev SA, Clements NJ, Cohen SJ, Caudill JD, et al. Characterization of hepatitis E virus (HEV) from Algeria and Chad by partial genome sequence. J Med Virol. 1997;53:340-7.
Engle RE, Yu C, Emerson SU, Meng XJ, Purcell RH. Hepatitis E virus (HEV) capsid antigens derived from viruses of human and swine origin are equally efficient for detecting anti-HEV by enzyme immunoassay. J Clin Microbiol. 2002;40:4576-80. http://dx.doi.org/10.1128/JCM. 40.12.4576-4580.2002
Lu L, Li C, Hagedorn CH. Phylogenetic analysis of global hepatitis E virus sequences: Genetic diversity, subtypes and zoonosis. Rev Med Virol. 2006;16:5-36.
Colson P, Brunet P, Lano G, Moal V. Hepatitis E virus genotype 4 in Southeastern France: Still around. Liver Int. 2015. http://dx.doi.org/10.1111/liv.12924
Monne I, Ceglie L, Di Martino G, Natale A, Zamprogna S, Morreale A, et al. Hepatitis E virus genotype 4 in a pig farm, Italy, 2013. Epidemiol Infect. 2015;143:529-33. http://dx.doi.org/10.1017/S0950268814001150
Hakze-van der Honing RW, van Coillie E, Antonis AF, van der Poel WH. First isolation of hepatitis E virus genotype 4 in Europe through swine surveillance in the Netherlands and Belgium. PLoS One. 2011;6:e22673. http://dx.doi.org/10.1371/journal.pone.0022673
Okamoto H. Genetic variability and evolution of hepatitis E virus. Virus Res. 2007;127:216-28. http://dx.doi.org/10. 1016/j.virusres.2007.02.002
Oliveira-Filho EF, Koenig M, Thiel H-J. Genetic variability of HEV isolates: Inconsistencies of current classification. Vet Microbiol. 2013;165:148-54. http://dx.doi.org/10.1016/j.vetmic.2013.01.026
Dai X, Dong C, Zhou Z, Liang J, Dong M, Yang Y, et al. Hepatitis E virus genotype 4, Nanjing, China, 2001-2011. Emerg Infect Dis.2013;19:1528-30. http://dx.doi.org/10.3201/eid1909.130013
Poddar U, Thapa BR, Prasad A, Singh K. Changing spectrum of sporadic acute viral hepatitis in Indian children. J Trop Pediatr. 2002;48:210-3. http://dx.doi.org/10.1093/tropej/48.4.210
Singh A, Singh S, Ansari MA, Irshad M. Co-infectivity of hepatitis B virus and hepatitis E virus. BMC Infect Dis. 2012;12(Supl.1):P1. http://dx.doi.org/10.1186/1471-2334-12-S1-P1
Rao P, Shenoy MS, Baliga S, Joon A. Prevalence of HAV and HEV in the patients presenting with acute viral hepatitis. Indian J Med Microbiol. 2015;33(Suppl.):102-5. http://dx.doi.org/10.4103/0255-0857.150908
Betancur C, Mejía M, Portillo S. Seroprevalencia de hepatitis E en trabajadores de fincas porcícolas del Valle de Aburrá, 2011-2012. Acta Médica Colombiana. 2013;38: 68-70.
Peláez D, Hoyos MC, Rendón JC, Mantilla C, Ospina MC, Cortés-Mancera F, et al. Infección por el virus de la hepatitis E en pacientes con diagnóstico clínico de hepatitis viral en Colombia. Biomédica. 2014;34:354-65. http://dx.doi.org/10.7705/biomedica.v34i3.2236
Wang Y, Ling R, Erker JC, Zhang H, Li H, Desai S, et al. A divergent genotype of hepatitis E virus in Chinese patients with acute hepatitis. J Gen Virol. 1999;80:169-77. http://dx.doi.org/10.1099/0022-1317-80-1-169
Zhai L, Dai X, Meng J. Hepatitis E virus genotyping based on full-length genome and partial genomic regions. Virus Res. 2006;120:57-69. http://dx.doi.org/10.1016/j.virusres.2006.01.013
Rodríguez-Lay LdeA, Quintana A, Montalvo-Villalba MC, Leimos G, Corredor MB, Moreno AG, et al. Dual infection with hepatitis A and E viruses in outbreaks and in sporadic clinical cases: Cuba 1998-2003. J Med Virol. 2008;80:798-802. http://dx.doi.org/10.1002/jmv.21147
Kumar A, Yachha SK, Poddar U, Singh U, Aggarwal R. Does co-infection with multiple viruses adversely influence the course and outcome of sporadic acute viral hepatitis in children? J Gastroenterol Hepatol. 2006;21:1533-7. http://dx.doi.org/10.1111/j.1440-1746.2006.04509.x
Zaki Mel S, Salama OS, Mansour FA, Hossein S. Hepatitis E virus coinfection with hepatotropic viruses in Egyptian children. J Microbiol Immunol Infect. 2008;41:254-8.
Pisanti FA, Coppola A, Galli C. Association between hepatitis C and hepatitis E viruses in southern Italy. Lancet. 1994;344:746-7.
Arankalle VA, Chobe LP. Retrospective analysis of blood transfusion recipients: Evidence for post-transfusion hepatitis E. Vox Sang. 2000;79:72-4. http://dx.doi.org/10. 1159/000031215
Boxall E, Herborn A, Kochethu G, Pratt G, Adams D, Ijaz S, et al. Transfusion-transmitted hepatitis E in a ‘nonhyperendemic’ country. Transfus Med. 2006;16:79-83. http://dx.doi.org/10.1111/j.1365-3148.2006.00652.x
Colson P, Coze C, Gallian P, Henry M, De Micco P, Tamalet C. Transfusion-associated hepatitis E, France. Emerg Infect Dis. 2007;13:648-9. http://dx.doi.org/10.3201/eid1304.061387
Haim-Boukobza S, Ferey MP, Vetillard AL, Jeblaoui A, Pelissier E, Pelletier G, et al. Transfusion-transmitted hepatitis E in a misleading context of autoimmunity and drug-induced toxicity. J Hepatol. 2012;57:1374-8. http://dx.doi.org/10.1016/j.jhep.2012.08.001.
Matsubayashi K, Nagaoka Y, Sakata H, Sato S, Fukai K, Kato T, et al. Transfusion-transmitted hepatitis E caused by apparently indigenous hepatitis E virus strain in Hokkaido, Japan. Transfusion. 2004;44:934-40. http://dx.doi.org/10. 1111/j.1537-2995.2004.03300.x
Matsubayashi K, Kang JH, Sakata H, Takahashi K, Shindo M, Kato M, et al. A case of transfusion-transmitted hepatitis E caused by blood from a donor infected with hepatitis E virus via zoonotic food-borne route. Transfusion. 2008;48:1368-75. http://dx.doi.org/10.1111/j.1537-2995. 2008.01722.x
Tolosa N. Hepatitis B, C y coinfección hepatitis B-Delta. Fecha de consulta: 16 de septiembre de 2015. Disponible en: http://www.ins.gov.co/lineas-de-accion/subdireccion-vigilancia/sivigila/protocolos%20sivigila/pro%20hepatitis% 20b%20c%20y%20delta.pdf
Ganova-Raeva L, Punkova L, Campo DS, Dimitrova Z, Skums P, Vu NH, et al. Cryptic hepatitis B and E in patients with acute hepatitis of unknown etiology. J Infect Dis. 2015;212:1962-9. http://dx.doi.org/10.1093/infdis/jiv315
Kamar N, Dalton HR, Abravanel F, Izopet J. Hepatitis E virus infection. Clin Microbiol Rev. 2014;27:116-38. http://dx.doi.org/10.1128/CMR.00057-13
Rodríguez-Frías F, Jardi R, Buti M. Hepatitis E: virología molecular, epidemiología y patogénesis. Enferm Infecc Microbiol Clin. 2015;30:624-34. http://dx.doi.org/10.1016/j.eimc.2012.01.014
Pillonel J, Gallian P, Sommen C, Couturier E, Piquet Y, Djoudi R, et al. Assessment of a transfusion emergent risk: The case of HEV. Transfus Clin Biol. 2014;21:162-6. http://dx.doi.org/10.1016/j.tracli.2014.07.004
Gallian P, Piquet Y, Assal A, Djoudi R, Chiaroni J, Izopet J, et al. Hepatitis E virus: Blood transfusion implications. Transfus Clin Biol. 2014;21:173-7. http://dx.doi.org/10. 1016/j.tracli.2014.07.007
Guzmán B, Nava G, Mejía A, Soler J. Estado de la vigilancia de la calidad del agua para consumo humano en Colombia - 2013. Fecha de consulta: 16 de septiembre de 2015. Disponible en: http://www.ins.gov.co/sivicap/Normatividad/Estado%20de%20la%20vigilancia%20de%20la%20calidad%20del%20agua%202013.pdf
Contreras M, González K. El acceso al agua para consumo humano en Colombia. Fecha de consulta: 16 de septiembre de 2015. Disponible en: http://www.economiainstitucional.com/esp/vinculos/pdf/No29/myanez29.pdf
Folleco A. Informe final hepatitis A, Colombia, 2013. Fecha de consulta: 16 de septiembre de 2015. Disponible en: http://www.ins.gov.co:81/lineas-de-accion/Subdireccion-Vigilancia/Informe%20de%20Evento%20Epidemiolgico/HEPATITIS%20A%202013.pdf
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