Methicillin-sensitive Staphylococcus aureus isolates related to USA300 clone: Origin of community-genotype MRSA in Colombia?

Javier Antonio Escobar-Pérez, Betsy Esperanza Castro, Ricaurte Alejandro Márquez-Ortiz, Sebastián Gaines, Bibiana Chavarro, Jaime Moreno, Aura Lucía Leal, Natasha Vanegas, .

DOI: https://doi.org/10.7705/biomedica.v34i0.1661
Keywords: Methicillin-resistant Staphylococcus aureus, infection, Colombia.
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Abstract

Introduction: USA300 is a genetic lineage found both in methicillin-resistant (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) isolates. In Colombia, hospital and community MRSA infections are caused by a USA300-related community genotype MRSA (CG-MRSA) clone. The genetic origin of this clone is unknown yet.

Objective: To identify and characterize methicillin-resistant (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates in order to improve the information about the origin of the CG-MRSA isolates in Colombia.

Materials and methods: USA300-related MSSA isolates were detected and characterized from a study of 184 S. aureus isolates (90 MRSA and 94 MSSA) recovered from infections. The genetic relatedness of the isolates was established by means of pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and protein A gene typification (spa typing).

Results: Among 184 isolates, 27 (14.7%) showed molecular characteristics and genetic relationship with the USA300 clone, of which 18 were MRSA and nine were MSSA. All USA300-related MRSA harbored Staphylococcal cassette chromosome mec (SCCmec) IVc (3.1.2). In the MSSA isolates, SCCmec remnants or attB duplicate sites were not detected.

Conclusions: In Colombia, the CG-MRSA isolates probably originated in the dissemination of an USA300-related MSSA clone which later acquired SCCmec IVc.

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  • Javier Antonio Escobar-Pérez Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C., Colombia
  • Betsy Esperanza Castro Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C., Colombia
  • Ricaurte Alejandro Márquez-Ortiz Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C., Colombia
  • Sebastián Gaines Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C., Colombia
  • Bibiana Chavarro Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C., Colombia
  • Jaime Moreno Grupo de Microbiología, Dirección de Investigación en Salud Pública, Subdirección de Investigación Científica y Tecnológica, Instituto Nacional de Salud, Bogotá, D.C., Colombia
  • Aura Lucía Leal Grupo para el Control de la Resistencia Bacteriana en Bogotá, Universidad Nacional de Colombia, Bogotá, D.C., Colombia
  • Natasha Vanegas Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, D.C., Colombia I3 Institute, Faculty of Science, University of Technology Sydney, Sydney, Australia
How to Cite
1.
Escobar-Pérez JA, Castro BE, Márquez-Ortiz RA, Gaines S, Chavarro B, Moreno J, et al. Methicillin-sensitive Staphylococcus aureus isolates related to USA300 clone: Origin of community-genotype MRSA in Colombia?. Biomed. [Internet]. 2014 Apr. 1 [cited 2025 Apr. 5];34(Sup1):124-36. Available from: https://revistabiomedicaorg.biteca.online/index.php/biomedica/article/view/1661

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Published
2014-04-01
Issue
Vol. 34 (2014): Abril, Suplemento 1, Resistencia bacteriana
Section
Original articles

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