Association of VDR gene ApaI polymorphism with obesity in Iranian population
Abstract
Introduction: Identifying obesity risk factors as a health problem facing communities is crucial given its complexity. The vitamin D receptor gene has been reported as a possible cause of this disease.
Objective: To study the association of the VDR gene ApaI, BsmI, and TaqI polymorphisms with obesity in an Iranian population.
Material and methods: We analyzed the genotypes of 348 obese (BMI≥30 kg/m2) and 320 non-obese people (BMI: 18.5-24.9 kg/m2) using PCR-RFLP. We measured FBS, TG, total cholesterol, and HDL and LDL cholesterol levels in an automatic biochemical analyzer.
Results: We found significantly higher BMI, FBS, and TG levels in the obese group compared to the control. In the obese individuals, the frequency of genotype AA was 47.1% and that of the combined Aa+aa genotype, 52.9% while in the control group they were 30% and 70%, respectively (p=0.024, 95% confidence interval (CI)=1.100-3.933, odds ratio (OR)=2.08). A and a alleles frequencies for the ApaI polymorphism were statistically significant in the two groups (allele A vs. a; p=0.017). No significant relationship was observed between TaqI genotypes and alleles in the control and obese subjects.
Conclusion: We found that VDR ApaI (rs7975232 C/A) polymorphism appeared to be a risk factor for obesity. Especially, the A allele and the AA genotype in ApaI were associated with the obesity phenotypes.
Downloads
References
Walley AJ, Asher JE, Froguel P. The genetic contribution to non-syndromic human obesity. Nat Rev Genet. 2009;10:431-42. https://doi.org/10.1038/nrg2594
Kopelman P. Health risks associated with overweight and obesity. Obes Rev. 2007;8(Suppl.1):13-17. https://doi.org/10.1111/j.1467-789X.2007.00311.x
World Health Organization W. Obesity: Preventing and managing the global epidemic. WHO Technical Report Series 894. 894th edition. Geneva: WHO; 2000.
Blum M, Dolnikowski G, Seyoum E, Harris SS, Booth SL, Petersonet J, et al. Vitamin D (3) in fat tissue. Endocrine. 2008;33:90-4. https://doi.org/10.1007/s12020-008-9051-4
Hypponen E, Power C. Vitamin D status and glucose homeostasis in the 1958 British birth cohort: The role of obesity. Diabetes Care. 2006;29:2244-6. https://doi.org/10.2337/dc06-0946
Wortsman J, Matsuoka LY, Chen TC, Lu Z, Holick MF. Decreased bioavailability of vitamin D in obesity. Am J Clin Nutr. 2000;72:690-3. https://doi.org/10.1093/ajcn/72.3.690
Uitterlinden AG, Fang Y, van Meurs JB, Pols HA, van Leeuwen JP. Genetics and biology of vitamin D receptor polymorphisms. Gene. 2004;338:143-56. https://doi.org/10.1016/j.gene.2004.05.014
Tabrizi JS, Sadeghi-Bazargani H, Farahbakhsh M, Nikniaz L, Nikniaz Z. Prevalence and associated factors of overweight or obesity and abdominal obesity in Iranian population: A population-based study of Northwestern Iran. Iran J Public Health. 2018;47:1583-92.
Mirzazadeh A, Sadeghirad B, Haghdoost AA, Bahreini F, Rezazadeh Kermani M. The prevalence of obesity in Iran in recent decade; a systematic review and meta-analysis study. Iran J Public Health. 2009; 38: 1-11.
Faghfouri AH, Faghfuri E, Maleki V, Payahoo L, Balmoral A, Khaje Bishak Y. A comprehensive insight into the potential roles of VDR gene polymorphism in obesity: A systematic review. Arch Physiol Biochem. 2020;1-13. https://doi.org/10.1080/13813455.2020.1788097
Vasilopoulos Y, Sarafidou T, Kotsa K, Papadimitriou M, Goutzelas Y, Stamatis C, et al. VDR TaqI is associated with obesity in the Greek population. Gene. 2013;512:237-9. https://doi.org/10.1016/j.gene.2012.10.044
Chen X, Wang W, Wang Y, Han X, Gao L. Vitamin D receptor polymorphisms associated with susceptibility to obesity: A meta-analysis. Med Sci Monit. 2019;25:8297-305. https://doi.org/10.12659/MSM.915678
World Health Organization. Obesity and overweight. 2013. Available from: https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight
Meamar R, Javadirad SM, Chitsaz N, Asadian M, Kazemi M, Ostadsharif M. Vitamin D receptor gene variants in Parkinson’s disease patients. Egypt J Med Hum Genet. 2017;18:225-30. https://doi.org/10.1016/j.ejmhg.2016.08.004
Khammissa RA, Fourie J, Motswaledi MH, Ballyram R, Lemmer J, Feller L. The biological activities of vitamin D and its receptor in relation to calcium and bone homeostasis, cancer, immune and cardiovascular systems, skin biology, and oral health. Biomed Res Int. 2018;2018. https://doi.org/10.1155/2018/9276380
Fleet JC. The role of vitamin D in the endocrinology controlling calcium homeostasis. Mol Cell Endocrinol. 2017;15:36-45. https://doi.org/10.1016/j.mce.2017.04.008
Bid HK, Mishra DK, Mittal RD. Vitamin-D receptor (VDR) gene (Fok-I, Taq-I and Apa-I) polymorphisms in healthy individuals from north Indian population. Asian Pac J Cancer Prev. 2005;6:147-52.
Zmuda JM, Cauley JA, Ferrell RE. Molecular epidemiology of vitamin D receptor gene variants. Epidemiol Rev. 2000;22:203-17. https://doi.org/10.1093/oxfordjournals.epirev.a018033
Ogunkolade BW, Boucher BJ, Prahl JM, Bustin SA, Burrin JM, Noonan K, et al. Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians. Diabetes. 2002;51:2294-300. https://doi.org/10.2337/diabetes.51.7.2294
Al-Daghri NM, Guerini FR, Al-Attas OS, Alokail MS, Alkharfy KM, et al. Vitamin D receptor gene polymorphisms are associated with obesity and inflammosome activity. PLoS ONE. 2014;9:e102141. https://doi.org/10.1371/journal.pone.0102141
Rahmadhani R, Zaharan NL, Mohamed Z, Moy FM, Jalaludin MY. The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country. PLoS ONE. 2017;12:e0178695. https://doi.org/10.1371/journal.pone.0178695
Ferrarezi DAF, Bellili-Munoz N, Nicolau C, Cheurfa N, Guazzelli IC, Frazzatto E, et al. Allelic variations in the vitamin D receptor gene, insulin secretion and parents’ heights are independently associated with height in obese children and adolescents. Metabolism. 2012;61:1413-21. https://doi.org/10.1016/j.metabol.2012.03.018
Correa-Rodríguez M, Carrillo-Ávila JA, Schmidt-RioValle J, González-Jiménez E, Vargas S, Martín J, et al. Genetic association analysis of vitamin D receptor gene polymorphisms and obesity-related phenotypes. Gene. 2018;15:51-6. https://doi.org/10.1016/j.gene.2017.10.029
Al-Hazmi AS, Al-Mehmadi MM, Al-Bogami SM, Shami AA, Al-Askary AA, Alomery AM, et al. Vitamin D receptor gene polymorphisms as a risk factor for obesity in Saudi men. Electronic Physician. 2017;9:5427-33. https://doi.org/10.19082/5427
Ostadsharif M, Rashidi-Khorasgani F. The relationship of FokI polymorphism in vitamin D receptor (VDR) gene and obesity. J Isfahan Med Sch. 2015;33:350.
Jakubowska-Pietkiewicz E, Klich I, Fendler W, Mlynarski W, Chlebna-Sokol D. Effect of vitamin D receptor gene (VDR) polymorphism on body height in children-own experience. Postepy Hig Med Dosw (Online). 2013;67:873-8. https://doi.org/10.5604/17322693.1063747
Nosratabadi R, Arababadi MK, Salehabad VA, Shamsizadeh A, Mahmoodi M, Sayadi AR, et al. Polymorphisms within exon 9 but not intron 8 of the vitamin D receptor are associated with the nephropathic complication of type-2 diabetes. Int J Immunogenet. 2010;37:493-7. https://doi.org/10.1111/j.1744-313X.2010.00953.x
Tokita A, Matsumoto H, Morrison NA, Tawa T, Miura Y, Fukamauchi K, et al. Vitamin D receptor alleles, bone mineral density and turnover in premenopausal Japanese women. J Bone Miner Res. 1996;11:1003-9. https://doi.org/10.1002/jbmr.5650110718
Fountas L, Moutsatsou P, Kastanias I, Tamouridis N, Tzanela M, Anapliotou M, et al. The contribution of vitamin d receptor gene polymorphisms in osteoporosis and familial osteoporosis. Osteoporos Int. 1999;10:392-8. https://doi.org/10.1007/s001980050245
Li L, Wu B, Liu J-Y, Yang L-B. Vitamin D receptor gene polymorphisms and type 2 diabetes: A meta-analysis. Arch Med Res. 2013;44:235-41. https://doi.org/10.1016/j.arcmed.2013.02.00
Kamel MM, Fouad SA, Salaheldin O, Abd El-Razek AA, Abd El-Fatah AI. Impact of vitamin D receptor gene polymorphisms in pathogenesis of type-1 diabetes mellitus. Int J Clin Exp Med. 2014;7:5505-10.
Fatma H, Abdul SN. Association of vVitamin D receptor gene BsmI polymorphism with type 2 diabetes mellitus in Pakistani population. Afri Health Sci. 2019;19 2164-71. https://doi.org/10.4314/ahs.v19i2.41
Sikander M. Relationship of ApaI VDR gene polymorphism to type 2 diabetes mellitus in Pakistani population. Electronic J Biol. 2017;13:338-42.
Santos BR, Lecke SB, Spritzer PM. Apa-I polymorphism in VDR gene is related to metabolic syndrome in polycystic ovary syndrome: A cross-sectional study. Reprod Biol Endocrinol. 2018;16:38. https://doi.org/10.1186/s12958-018-0355-9
Angel B, Lera L, Márquez C, Albala C. The association of VDR polymorphisms and type 2 diabetes in older people living in community in Santiago de Chile. Nutr Diabetes. 2018;8:31. https://doi.org/10.1038/s41387-018-0038-9
Mohamed S, El-Askary A. Vitamin D receptor gene polymorphism among Egyptian obese children. Asian J Clin Nutr. 2017;9:24-9. https://doi.org/10.3923/ajcn.2017.24.29
Some similar items:
- Nelsy Loango, Martha Lucía Gallego, Beatriz Restrepo, Patricia Landázuri, Gender, age and plasma lipids differences associated with apolipoprotein E polymorphism in school children , Biomedica: Vol. 29 No. 3 (2009)
- Liliana Franco-Hincapié, Constanza Elena Duque, María Victoria Parra, Natalia Gallego, Alberto Villegas, Andrés Ruiz-Linares, Gabriel Bedoya, Association between polymorphism in uncoupling proteins and type 2 diabetes in a northwestern Colombian population , Biomedica: Vol. 29 No. 1 (2009)
- Ana Victoria Valencia, Ana Lucía Páez, María Elena Sampedro, Clara Ávila, Julio Cesar Cardona, Catalina Mesa, Lina Galvis, Jaime Carrizosa, Mauricio Camargo, Andrés Ruíz, William Cornejo, Gabriel Bedoya, Evidence for association and epistasis between the genetic markers SLC6A4 and HTR2A in autism etiology , Biomedica: Vol. 32 No. 4 (2012)
- Ney Callas, Elpidia Poveda, César Baracaldo, Patricia Hernández, Carlina Castillo, Martha Guerra, Genetic polymorphism of the E apolipoprotein in school age children: comparison with levels of plasma lipids and apolipoproteins , Biomedica: Vol. 27 No. 4 (2007)
- Claudia Liliana Durán, Olga Lucía Morales, Sandra Johanna Echeverri, Mario Isaza, Beta globin haplotypes in hemoglobin S carriers in Colombia , Biomedica: Vol. 32 No. 1 (2012)
- Nora Cardona-Castro, Miryan Sánchez-Jiménez, Winston Rojas, Gabriel Bedoya-Berrío, IL-10 gene promoter polymorphisms and leprosy in a Colombian population sample , Biomedica: Vol. 32 No. 1 (2012)
- Andrés Javier Quiroga, Antonio Huertas, Alba Lucía Cómbita, María Mercedes Bravo, Variation in the number of EPIYA-C repeats in CagA protein from Colombian Helicobacter pylori strains and its ability to induce hummingbird phenotype in gastric epithelial cells , Biomedica: Vol. 30 No. 2 (2010)
- Claudia Ayala, Reggie García, Edith Cruz, Karol Prieto, Marta Bermúdez, Homocysteine levels and polymorphisms of MTHFR and CBS genes in Colombian patients with superficial and deep venous thrombosis , Biomedica: Vol. 30 No. 2 (2010)
- Carlos Isaza, Leonardo Beltrán, Julieta Henao, Gloria Porras, Alfredo Pinzón, Álvaro Vallejos, Jorge Machado, Genetic and bioenvironmental factors associated with warfarin response in Colombian patients , Biomedica: Vol. 30 No. 3 (2010)
- Olga María Moreno, Clara Isabel González, Diego Luis Saaibi, William Otero, Reynaldo Badillo, Javier Martín, Gerardo Ramírez, Polymorphisms of IL-10 gene promoter and rheumatoid arthritis in a Colombian population , Biomedica: Vol. 27 No. 1 (2007)
| Article metrics | |
|---|---|
| Abstract views | |
| Galley vies | |
| PDF Views | |
| HTML views | |
| Other views | |
