Antimicrobial activity of endophytic fungi from the medicinal plants Mammea americana (Calophyllaceae) and Moringa oleifera (Moringaceae)
Abstract
Introduction: Infectious diseases represent one of the leading causes of death worldwide. Considering the growing global challenge of antimicrobial resistance, research into new sources of potentially effective antimicrobial agents from natural origins is of great importance for world health.
Objective: To evaluate the antimicrobial activity of endophytic fungi from Mammea americana and Moringa oleifera upon Staphylococcus aureus (ATCC 29213), S. aureus (resistant strain USb003), Escherichia coli (ATCC 25922), and E. coli (resistant strain USb007).
Materials and methods: We isolated endophytic fungi from the leaves, seeds, and stems of the two plants under study. We evaluated their antimicrobial activity through the formation of sensitivity haloes in dual tests in vitro, as well as in trials using crude ethanolic extracts from the endophytes. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and cytotoxicity o the substances were analyzed.
Results: Three ethanolic extracts of Penicillium sp., Cladosporium (001), and Cladosporium (002) exhibited the greatest inhibition halos in sensitive and resistant strains of E. coli and S. aureus. The MIC and CBM found were statistically significant (p≤0.05) compared with the gentamicin control. Furthermore, the cytotoxicity test results of CC50>1,000 demonstrated that the endophytic fungi studied exhibit bactericidal characteristics without causing unintended damage.
Conclusion: The endophytic fungi M. oleifera and M. americana represent a source of active secondary metabolites with antimicrobial and non-toxic properties. In light of these findings, further research should proceed with chemical identification of the compounds and the study of their mechanisms of action, especially given the paucity of current scientific knowledge concerning the isolation of endophytes in these plants.
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von Wintersdorff CJH, Penders J, van Niekerk JM, Mills ND, Majumder S, van Alphen LB, et al. Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer. Front Microbiol. 2016;7:173. https://doi.org/10.3389/fmicb.2016.00173
Bisht D, Owais M, Venkatesan K. Potential of plant‐derived products in the treatment of mycobacterial infections. En: Ahmad I, Aqil F, Owais M, editors. Modern phytomedicine: Turning medicinal plants into drugs. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA; 2006. p. 295-6. https://doi.org/10.1002/9783527609987.ch14
Alvin A, Miller KI, Neilan BA. Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds. Microbiol Res. 2014;169:483-95. https://doi.org/10.1016/j.micres.2013.12.009
Bary A. Morphologie und Physiologie der Pilze, Flechten und Myxomyceten. W. Leipzig : W. Engelmann; 1866. p. 21-2. https://doi.org/10.5962/bhl.title.120970
Wilson D. Endophyte: The evolution of a term, and clarification of its use and definition. Oikos.1995;73:274-6. https://doi.org/10.2307/3545919
Schulz BJ, Boyle C. The endophytic continuum review. Mycol Res. 2005;109:661-86. https://doi.org/10.1017/S095375620500273X
Kusari S, Hertweck C, Spiteller M. Chemical ecology of endophytic fungi: Origins of secondary metabolites. Chem Biol. 2012;19:792-8. https://doi.org/10.1016/j.chembiol.2012.06.004
Heinig U, Scholz S, Jennewein S. Getting to the bottom of Taxol biosynthesis by fungi. Fungal Divers. 2013;60:161-70. https://doi.org/10.1007/s13225-013-0228-7
Guo B, Dai JR, Ng S, Huang Y, Leong C, Ong W, Carté BK. Cytonic acids A and B: Novel tridepside inhibitors of hCMV protease from the endophytic fungus Cytonaema species. J Nat Prod. 2000;63:602-4. https://doi.org/10.1021/np990467r
Mahecha VG, Ovalle EA, Camelo SD, Rozo FA, Barrero BD. Vegetación del territorio CAR: 450 especies de sus llanuras y montañas. Fecha de consulta: 18 de noviembre de 2017. Disponible en: http://biblioteca.humboldt.org.co/cgi-bin/koha/opac-detail.pl?biblionumber=5243&query_desc=au%2Cwrdl%3A%20Barrero%20
Little EL, Woodbury R, Wadsworth FH. Trees of Puerto Rico and the Virgin Islands. Fecha de consulta: 20 de noviembre de 2017. Disponible en: http://edicionesdigitales.info/biblioteca/arbolesprvi2engl.pdf
Yang H, Jiang B, Reynertson KA, Basile MJ, Kennelly EJ. Comparative analyses of bioactive Mammea Coumarins from seven parts of Mammea americana by HPLC-PDA with LC-MS. J Agric Food Chem. 2006;54:4114-20. https:/doi.org/10.1021/jf0532462
Sánchez-Peña YA, Martínez-Ávila GC, Sinagawa-García SR, Vázquez-Rodríguez JA. Moringa oleifera; importancia, funcionalidad y estudios involucrados. Revista Científica de la Universidad Autónoma de Coahuila. 2013;5:25-30.
Fischer HW. Moringa oleifera. Magic, myth or miracle. Salem-USA: Britannia Printing; 2012. p. 50-136.
Alfaro N, Martínez W. Uso potencial de la Moringa (Moringa oleifera Lam) para la producción de alimentos nutricionalmente mejorados. Fonacyt. Fecha de consulta: 18 de noviembre de 2017. Disponible en: https://docplayer.es/89330773-Uso-potencial-de-la-moringa-moringaoleifera-lam-para-la-produccion-de-alimentos-nutricionalmente-mejorados.html
Souza IF, Napoleão TH, de Sena KX, Paiva PM, de Araújo JM, Coelho LC. Endophytic microorganisms in leaves of Moringa oleifera collected in three localities at Pernambuco State, Northeastern Brazil. Microbiology Research Journal International. 2016;3:1-7 https://doi.org/10.9734/BMRJ/2016/24722
Zhao Z, Wang Q, Wang K, Brian K, Liu C, Gu Y. Study of the antifungal activity of Bacillus vallismortis ZZ185 in vitro and identification of its antifungal components. Bioresour Technol. 2010;101:292-7. https://doi.org/10.1016/j.biortech.2009.07.071
Echavarría A, D’ Armas H, Matute NL, Jaramillo C, Rojas-de-Astudillo L, Benítez R. Evaluation of antioxidant capacity and secondary metabolites of sixteen medicinal plants extracts. Ciencia Unemi. 2016;9:29-35.
Ahmedkhan H, Ahmad A, Mehboob R. Nosocomial infections and their control strategies. Asian Pac J Trop Biomed. 2015; 5509-14. https://doi.org/10.1016/j.apjtb.2015.05.001
Blanco J, Blanco M, Blanco JE, Mora A, Alonso MP, González EA, et al. Enterobacterias: características generales. Género Escherichia. En: Vadillo S, Piriz S, Mateos E, editores. Manual de Microbiología Veterinaria. Madrid: McGraw-Hill Interamericana; 2002. p. 301-25.
de la Hoz FJ, Santiago L. Infección urinaria recurrente en la mujer posmenopáusica. Revista Colombiana de Menopausia. 2018:19:3-4.
Pigrau C. Infecciones del tracto urinario nosocomiales. Enferm Infect Microbiol Clin. 2013;31:614-24. https://doi.org/10.1016/j.eimc.2012.11.015
Schulz B, Boyle C. The endophytic continuum. Mycol Res. 2005;109:661-86. https://doi.org/10.1017/S095375620500273X
Olaechea PM, Insaustib J, Blanco A, Luque P. Epidemiología e impacto de las infecciones nosocomiales. Medicina Intensiva. 2010;34:256-67. https://doi.org/10.1016/j.medin.2009.11.013
Gil de MM. Staphylococcus aureus: microbiología y aspectos moleculares de la resistencia a meticilina. Rev Chil Infectol. 2000;17:145-52. https://doi.org/10.4067/S0716-10182000000200010
Carroll K, Hobden J.A, Miller S, Morse S, Mietzner T, Detrick B, et al. Jawetz Melnick & Adelbergs Medical Microbiology. 27th edition. New York: Mc Graw Hill Education; 2016. p. 363-94.
World Health Organization. Global strategy for containment of antimicrobial resistance. Geneve: WHO; 2018. p. 3-4. https://www.who.int/es/news-room/fact-sheets/detail/resistencia-a-los-antimicrobianos
Unterseher M, Schnittler M. Dilution-to-extinction cultivation of leaf-inhabiting endophytic fungi in beech (Fagus sylvatica L.) –different cultivation techniques influence fungal biodiversity assessment. Mycol Res. 2009;113:645-54. https://doi.org/10.1016/j.mycres.2009.02.002
Carbungco ES, Pedroche NB, Panes VA, De la Cruz TE. Identification and characterization of endophytic fungi associated with the leaves of Moringa oleifera Lam. Acta Horticulturae. 2017;1158:373-80. https://doi.org/10.17660/ActaHortic.2017.1158.42
Watanabe T. Pictorial Atlas of Soil and Seed Fungi: Morphologies of cultured fungi and key to species. Boca Raton: CRC Press Taylor & Francis Group; 2010. p. 426.
Mishra VK, Passari AK, Chandra P, Leo VV, Kumar B, Uthandi S, et al. Determination and production of antimicrobial compounds by Aspergillus clavatonanicus strain MJ31, an endophytic fungus from Mirabilis jalapa L. using UPLC-ESI-MS/MS and TD-GC-MS analysis. PloS One. 2017;12:e0186234. https://doi.org/10.1371/journal.pone.0186234
Liang H, Xing Y, Chen J, Zhang D, Guo S, Wang C. Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae). BMC Complement Altern Med. 2012;12:2-6. https://doi.org/10.1186/1472-6882-12-238
Dos Santos IP, da Silva LC, da Silva MV, de Araújo JM, Cavalcanti Mda S, Lima VL. Antibacterial activity of endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae). Front Microbiol. 2015;350:1-7. https://doi.org/10.3389/fmicb.2015.00350
Zhao J, Shan T, Mou Y, Zhou L. Plant-derived bioactive compounds produced by endophytic fungi. Mini Rev Med Chem. 2011;11:159-68. https://doi.org/10.2174/138955711794519492
Gond SK, Mishra A, Sharma VK, Verma SK, Kumar J, Kharwar RN, et al. Diversity and antimicrobial activity of endophytic fungi from isolated from Nyctanthes arbor-tristis, a well know medicinal plant of India. Mycoscience. 2011;53:113-21. https://doi.org/10.1007/S10267-011-0146-Z
Yu H, Zhang L, Li L, Zheng C, Guo L, Li W, et.al. Recent developments and future prospects of antimicrobial metabolites produced by endophytes. Microbiol Res. 2010;165:437-49. https://doi.org/10.1016/j.micres.2009.11.009
Idris A, Al-tahir L, Idris E. Antibacterial activity of endophytic fungi extracts from the medicinal plant Kigelia africana. Egypt Acad J Biolog Sci. 2013;5:1-9.
Rajeswari S, Umamaheswari S, Prasanth D.A, Rajamanikandan KC. Study of endophytic fungal community of Moringa oleifera from Omalur Region – Salem. Int J Pharm Sci Res. 2014;5:4887-92. https://doi.org/10.13040/IJPSR.0975-8232.5(11).4887-92
Sugijanto EN, Dorra LB. Antimicrobial activity of Cladosporium oxysporum endophytic fungus extract isolated from Aglaia odorata Lour. Indonesian Journal of Medicine. 2016;2:108-15. https://doi.org/10.26911/theijmed.2016.01.02.04
de Medeiros LS, Murgu M, de Souza AQ, Rodrigues FO. Antimicrobial depsides produced by Cladosporium uredinicola, an endophytic fungus isolated from Psidium guajava fruits. Helv Chim Acta. 2011;94:1077-84 https://doi.org/10.1002/hlca.201000387
Prihanto A, Firdaus M, Nurdiani R. Endophytic fungi isolated from Mangrove (Rhyzopora mucronata) and its antibacterial activity on Staphylococcus aureus and Escherichia coli. J Food Sci Eng. 2011;1:386-9
Dhanalakshmi R, Umamaheswari S, Sugandhi P, Prasanth DA. Biodiversity of the endophytic fungi isolated from Moringa oleifera of Yercaud hills. Int J Pharm Sci Res. 2013;4:1064-8. https://doi.org/10.13040/IJPSR.0975-8232.4(3).1064-68
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