Genetic characterization of the interleukin 1 β polymorphisms -511, -31 y +3954 in a Colombian population with dyspepsia
Keywords:
polymorphism, single nucleotide, interleukin-1beta, polymerase chain reaction, restriction fragment length, Helicobacter pylori, pathology
Abstract
Introduction. The human interleukin-1β gen (IL-1 β) polymorphisms such as -511, -31 and +3954 have been associated with the presence of gastric cancer, due to the inhibitor effect that this protein has on acid secretion in the stomach. Thisfacility can enhance the colonization and infection by agents like Helicobactor. pylori and the genesis of preneoplastic states that can lead to cancer development.Objective. Three polymorphisms of IL-1β (+3954, -511 and -31) will be genetically characterized and their frequencies established in a population of patients with gastric symptoms.
Materials and methods. Gastric antrum biopsies were obtained from 111 patients that showed signs of gastric disorder. A PCR was done to detect the H. pylori presence; a PCR using designed primers for specific regions was done to define the three polymorphic regions of IL-1β, and a RFLP was carried out using Aval, Alul and TaqI for the position -511, -231 and +3954 for each case.
Results. Helicobacter pylori was detected in 59.5% of the evaluated gastric while the histopathology study revealed that 82.9% of patients had some pathology. Characterization of polymorphic regions of IL-1β gen were joined to RFLP typing evidenced that all descfribed genotypes were present in the study population. However, patients with benign pathologies infected with H. pylori had a high frequency of the CC genotype (28.6%) in the -31 polymorphic regions.
Conclusion. No significant differences were found between the genotype frequenciess of the H. pylori-infected and the non-infected populations with one exception. The CC genotype in the -31 polymorphic region was associated with benign pathologies.
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References
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18. Palli D, Saieva C, Luzzi D, Masala G, Topa S, Sera F, et al. Interleukin-1 gene polymorphisms and gastric cancer risk in a high-risk Italian population. Am J Gastroenterol. 2005;100:1941-8.
19. Ruzzo A, Graziano F, Pizzagalli F, Santini D, Battistelli V, Panunzi D, et al. Interleukin 1β gene (IL-1β) and interleukin 1 receptor antagonist gene (IL-1RN) polymorphisms in Helicobacter pylori negative gastric cancer of intestinal and diffuse histotype. Ann Oncol. 2005;16:887-92.
20. Flórez O, Zafra G, Morillo C, Martín J, González CI. Interleukin-1 gene cluster polymorphism in Chagas disease in a Colombian case-control study. Hum Immunol. 2006;67:741-8.
21. Gómez LM, Camargo JF, Castiblanco J, Ruiz-Narváez EA, Cadena J, Anaya JM. Analysis of IL1B, TAP1, TAP2 and IKBL polymorphisms on susceptibility to tuberculosis. Tissue Antigens. 2006;67:290-6.
22. Bartchewsky W, Martini MR, Masiero M, Squassoni AC, Álvarez MC, Sady M, et al. Effect of Helicobacter pylori infection in IL-8, IL-1β and COX-2 expression in patients with chronic gastritis and gastric cancer. Scand J Gastroenterol. 2009;44:153-61.
23. Cañas M, Morán Y, Rivero MB, Bohórquez A, Villegas V, Redón Y, et al. Polimorfismo genético de interleuquina-1: asociación con cáncer gástrico en la población de alto riesgo del centroccidente de Venezuela. Rev Med Chil. 2009;137:63-70.
24. Achyut BR, Moorchung N, Mittal B. Genetic association of interleukin-1 haplotypes with gastritis and precancerous lesions in North Indianas. Clin Exp Med. 2008;8:23-9.
2. Dubois A, Borén T. Helicobacter pylori is invasive and it may be a facultative intracellular organism. Cell Microbiol. 2007;9:1108-16.
3. Scott Algood H, Cover T. Helicobacter pylori persistence: an overview of interactions between H. pylori and host immune defenses. Clin Microbiol Rev. 2006;19:597-613.
4. Rad R, Dossumbekoba A, Neu B, Lang R, Bauer S, Saur D, et al. Cytokine gene polymorphisms influence mucosal cytokine expression, gastric inflammation, and host specific colonization during Helicobacter pylori infection. Gut. 2004;53:1082-9.
5. Zhang D, Zhens H, Zhou Y, Tang X, Baojun Y, Li J. Association of IL 1beta gene polymorphism with cachexia from locally advanced gastric cancer. BMC Cancer. 2007;7:45.
6. Zeng Z, Hu J, Hu S, Pang R, Chen M, Sung J. Association of interleukin 1β gene polymorphism and gastric cancers in high and low prevalence regions in China. Gut. 2003;52:1684-9.
7. Beales I. Effect of Interleukin 1β on proliferation of gastric epitelial cells in culture. BMC Gastroenterol. 2002;2:2-8.
8. Vélez-Castrillón S, Camargo J, Correa P, Anaya J. Bases moleculares de la familia de la interleuquina 1. Revista Colombiana de Reumatología. 2004;11:11-39.
9. Montealegre CM, Jaramillo C, Bohórquez M, Montealegre G, Delgado M. Detección de Helicobacter pylori y caracterización del gen de la interleuquina-1β humana en pacientes de una población colombiana con enfermedades gastroduodenales. Revista Colombiana de Gastroenterología. 2008;23:40-4.
10. Di Giovine FS, Takhsh E, Blakemore AI, Duff GW. Single base polymorphism at -511 in the human interleukin-1b gene (IL1b). Hum Mol Genet. 1992;1:450.
11. Martínez JD, Henao S, Granados C. La gastritis crónica atrófica corporal y la edad. Revista Colombiana de Gastroenterología. 2007;22:17-22.
12. Bravo LE, Cortés A, Carrascal E, Jaramillo R, García LS, Bravo PE, et al. Helicobacter pylori: patología y prevalencia en biopsias gástricas en Colombia. Colombia Médica. 2003;34:124-31.
13. Piñol F, Paniagua M. Citoquinas, gastritis crónica y Helicobacter pylori. Rev Cubana Hematol Inmunol Hemoter. 2000;16:184-9.
14. Fox JG, Wang TC. Inflammation, atrophy, and gastric cancer. J Clin Invest. 2007;117:60-9.
15. El-Omar E, Carrington M, Chow W, McColl K, Yuan C, Rothman N, et al. Interleukin-1 polymorfisms associated with increased risk of gastric cancer. Nature. 2000;404:398-402.
16. Chun L, Xia H, Xie W, Ye M, Li J, Cheng H, et al. Association between inteleukin-1 gene polymorphisms and Helicobacter pylori infection in gastric carcinogenesis in a Chinese population. J Gastroenterol Hepatol. 2007;22:234-9.
17. García M, Lanas A, Santolaria S, Crusius A, Serrano M, Peña A. The polymorphic IL-1β and IL-1RN genes in the aetiopahogenisis of peptic ulcer. Clin Exp Immunol. 2001;125:368-75.
18. Palli D, Saieva C, Luzzi D, Masala G, Topa S, Sera F, et al. Interleukin-1 gene polymorphisms and gastric cancer risk in a high-risk Italian population. Am J Gastroenterol. 2005;100:1941-8.
19. Ruzzo A, Graziano F, Pizzagalli F, Santini D, Battistelli V, Panunzi D, et al. Interleukin 1β gene (IL-1β) and interleukin 1 receptor antagonist gene (IL-1RN) polymorphisms in Helicobacter pylori negative gastric cancer of intestinal and diffuse histotype. Ann Oncol. 2005;16:887-92.
20. Flórez O, Zafra G, Morillo C, Martín J, González CI. Interleukin-1 gene cluster polymorphism in Chagas disease in a Colombian case-control study. Hum Immunol. 2006;67:741-8.
21. Gómez LM, Camargo JF, Castiblanco J, Ruiz-Narváez EA, Cadena J, Anaya JM. Analysis of IL1B, TAP1, TAP2 and IKBL polymorphisms on susceptibility to tuberculosis. Tissue Antigens. 2006;67:290-6.
22. Bartchewsky W, Martini MR, Masiero M, Squassoni AC, Álvarez MC, Sady M, et al. Effect of Helicobacter pylori infection in IL-8, IL-1β and COX-2 expression in patients with chronic gastritis and gastric cancer. Scand J Gastroenterol. 2009;44:153-61.
23. Cañas M, Morán Y, Rivero MB, Bohórquez A, Villegas V, Redón Y, et al. Polimorfismo genético de interleuquina-1: asociación con cáncer gástrico en la población de alto riesgo del centroccidente de Venezuela. Rev Med Chil. 2009;137:63-70.
24. Achyut BR, Moorchung N, Mittal B. Genetic association of interleukin-1 haplotypes with gastritis and precancerous lesions in North Indianas. Clin Exp Med. 2008;8:23-9.
How to Cite
1.
Arango MT, Jaramillo C, Montealegre MC, Bohórquez MH, Delgado M del P. Genetic characterization of the interleukin 1 β polymorphisms -511, -31 y +3954 in a Colombian population with dyspepsia. Biomed. [Internet]. 2010 Aug. 4 [cited 2025 Apr. 9];30(2):199-206. Available from: https://revistabiomedicaorg.biteca.online/index.php/biomedica/article/view/183
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