Production of mouse recombinant JAK2 protein
Keywords:
JAK2, growth hormone (GH), growth hormone receptor (GHR), baculovirus
Abstract
This work describes the production of JAK2 by recombinan, methods, using a baculovirus expression vector. Sf9 cells from the ovary tissue of Spodoptera frugiperda were infected with an optimal virus dilution of 10plIml medium and harvest was done at 72 h postinfection. Approximately 5 x 107cells were infected, extracts were collected, ultrafiltered and subjected to ionic exchange chromatography on DEAE-Sephacel, and JAK2 was partially purified by a continuos ionic gradient (1 0-500 mM NaCI). JAK2 was identified by Western blot with a polyclonal antibody raised in rabbits against 758-776 residues from murine JAK2. Results showed that the protein obtained by this method is in phosphorylatedl activated state.Downloads
Download data is not yet available.
How to Cite
1.
Garzón RE, Flores A, Anzola C, Sánchez de Gómez M. Production of mouse recombinant JAK2 protein. Biomed. [Internet]. 2000 Mar. 1 [cited 2026 Jan. 12];20(1):10-7. Available from: https://revistabiomedicaorg.biteca.online/index.php/biomedica/article/view/1042
Some similar items:
- Wilson Mejía, Carlos Castro, Adriana Umaña, Clemencia de Castro, Tulia Riveros, Myriam Sánchez-Gómez, Insulin-like growth factor receptor I signaling in a breast cancer cell line , Biomedica: Vol. 30 No. 4 (2010)
- Ivonne Rubio, Alba Lucía Cómbita, Blanca Ortiz Reyes, María Cristina Navas, Hepatitis C virus core protein production and purification in a baculovirus expression system for biological assays. , Biomedica: Vol. 25 No. 1 (2005)
- María Fernanda Unigarro, Catalina Forero , Camila Céspedes , Neuropsychological and physical development of patients diagnosed with congenital hypothyroidism at the San Ignacio University Hospital between 2001 and 2017 , Biomedica: Vol. 42 No. Sp. 1 (2022): Mayo, Enfermedades crónicas en el trópico
Published
2000-03-01
Issue
Section
Original articles
| Article metrics | |
|---|---|
| Abstract views | |
| Galley vies | |
| PDF Views | |
| HTML views | |
| Other views | |
Escanea para compartir
